Protist Interactions and Seasonal Dynamics in the Coast of Yantai, Northern Yellow Sea of China as Revealed by Metabarcoding  

作　　者：FU Yingjun ZHENG Pengfei ZHANG Xiaoli ZHANG Qianqian JI Daode 

机构地区：[1]School of Ocean,Yantai University,Yantai 264003,China [2]Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China [3]Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China

出　　处：《Journal of Ocean University of China》2020年第4期961-974,共14页中国海洋大学学报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.31672251,31772413);the Youth Innovation Promotion Association,CAS(No.2019216);the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA23050303);the Key Research Project of Frontier Science,CAS(No.QYZDBSSW-DQC013-1).

摘　　要：Facilitated by the high-throughput sequencing(HTS)technique,the importance of protists to aquatic systems has been widely acknowledged in the last decade.However,information of protistan biotic interactions and seasonal dynamics is much less known in the coast ecosystem with intensive anthropic disturbance.In this study,year-round changes of protist community composition and diversity in the coastal water of Yantai,a city along the northern Yellow Sea in China,were investigated using HTS for the V4 region of 18S rDNA.The interactions among protist groups were also analyzed using the co-occurrence network.Data analyses showed that Alveolata,Chlorophyta,and Stramenopiles are the most dominant phytoplanktonic protists in the investigated coastal area.The community composition displayed strong seasonal variation.The abundant families Dino-Group-I-Clade-1 and Ulotrichales_X had higher proportions in spring and summer,while Bathycoccaceae exhibited higher ratios in autumn and winter.Alpha diversities(Shannon and Simpson)were the highest in autumn and the lowest in spring(ANOVA test,P<0.05).Nutrients(SiO42−,PO43−),total organic carbon(TOC),and pH seemed to drive the variation of alpha diversity,while temperature,PO43−and TON were the most significant factors influencing the whole protist community.Co-variance network analyses reveal frequent co-occurrence events among ciliates,chlorophytes and dinoflagellate,suggesting biotic interactions have been induced by predation,parasitism and mixotrophy.

关 键 词：coastal zone biotic interaction high-throughput sequencing northern Yellow Sea protist diversity seasonal dynamic 

分 类 号：P73[天文地球—海洋科学] Q178.53[生物学—水生生物学]