^1H NMR Quantification of DHA and EPA in Fish Oil  

作　　者：LV Jinxiao WANG Cong ZHANG Xiuli LV Zhihua YU Mingming 

机构地区：[1]School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266003,China [3]Key Laboratory of Glycoscience&Glycotechnology of Shandong Province,Qingdao 266003,China [4]Key Laboratory of Marine Drugs,Ministry of Education of China,Qingdao 266003,China

出　　处：《Journal of Ocean University of China》2020年第5期1193-1197,共5页中国海洋大学学报（英文版）

基　　金：supported by the Open Research Fund Program of Shandong Provincial Key Laboratory of Glycoscience&Glycotechnology (Ocean University of China);the Fundamental Research Funds for the Central Universities (Nos.201912008,201964019,and 201851025)。

摘　　要：Fish oil is a popular nutritional product consumed in China. The beneficial effects of fish oil have been attributed to docosahexaenoic acid(DHA) and eicosapentaenoic acid(EPA). Hence, it is crucial to develop a rapid and precise method to determine the contents of EPA and DHA in fish oil. In this study, a rapid and accurate proton nuclear magnetic resonance method for the quantification of DHA and EPA was developed. Dimethyl terephthalate was selected as an internal standard, and the signals at 2.391 ppm for DHA and at 1.697 ppm for EPA were chosen for the quantification. Validation of the method was performed in terms of specificity, precision, and stability. The results indicated that the method was precise and in line with the China Food and Drug Administration guidance. The method has been successfully applied to characterize fish oil capsules obtained from four pharmaceutical companies. This study indicated that the rapid ~1H NMR method is suitable for the quality control of fish oil.

关 键 词：^1H NMR EPA DHA fish oil quality control 

分 类 号：TS225.24[轻工技术与工程—粮食、油脂及植物蛋白工程]