Studies on the Attached Cultivation of Filamentous Oleaginous Microalga Tribonema minus  

作　　者：ZHANG Yan JI Chunli ZHOU Wenjun WANG Hui WANG Junfeng LIU Tianzhong 

机构地区：[1]Key Laboratory of Biofuels,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences(CAS),Qingdao 266101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Taigu 030801,China [4]Dalian National Laboratory for Clean Energy,Dalian 116023,China

出　　处：《Journal of Ocean University of China》2020年第3期691-699,共9页中国海洋大学学报（英文版）

基　　金：supported by the Strategic Priority Research Program of Chinese Academy of Sciences (Transformational Technologies for Clean Energy and Demonstration)(No.XDA21010211);the Shandong Provincial Natural Science Foundation (No.ZR2017QC007);the Youth Innovation Promotion Association, CAS。

摘　　要：Attached cultivation is a promising method for microalgal biomass production. Filamentous oleaginous microalga Tribonema minus(hereafter T. minus) has shown a remarkable potential for biofuel production in terms of its high lipid content. However, the strain has only been cultivated in suspended cultivation systems including open pond and closed photobioreactors. Here, we attempted to study the attached cultivation of T. minus, which might be helpful for its scale-up cultivation and industrial applications. As the results, the optimal conditions for T. minus growth in the attached biofilm are 200 μmol photons m^-2 s^-1 of light intensity and 5% of CO2, and the maximum biomass density of 223 g m^-2 has been achieved under the light intensity. The non-woven fabric as substratum was found as the best substratum in thin layer attached bioreactor, on which the average biomass productivity of T. minus is about(9.73 ± 2.19) g m^-2)d^-1. Furthermore, two attached bioreactor systems, rotary drum and rotation disc, were designed following the light dilution strategy and introduced into T. minus cultivation. The highest footprint areal biomass productivity of these two systems is 33 and 47.1 g m^-2 d^-1, respectively, much higher than that in suspended cultivation system. The results shows that T. minus can be cultured with attached cultivation method to improve its biomass productivity.

关 键 词：Tribonema minus attached culture biomass productivity light dilution 

分 类 号：S968.49[农业科学—水产养殖]