芍药苷对慢性高眼压大鼠RGCs保护作用的研究  

Protective effect of paeoniflorin on retinal ganglion cells in chronic intraocular hypertension rats

作　　者：张丹[1,2] 赵军 孙凤江[2] 张娟美 季亚男[1,2] ZHANG Dan;ZHAO Jun;SUN Fengjiang(Qingdao University,Qingdao 266000,China)

机构地区：[1]青岛大学,青岛266000 [2]山东省临沂市人民医院眼科,临沂276002

出　　处：《中国中医眼科杂志》2020年第4期239-243,共5页China Journal of Chinese Ophthalmology

基　　金：临沂市科技发展计划项目(201919098)。

摘　　要：目的探讨芍药苷对慢性高眼压大鼠模型视网膜神经节细胞(RGCs)凋亡的影响及作用机制。方法将30只SD大鼠随机分为假手术组,高眼压模型组及芍药苷治疗组,均以右眼作为实验眼。采用标准烧灼巩膜上静脉法建立慢性高眼压大鼠模型,假手术组仅剪开球结膜,不处理巩膜表层静脉。术后当日及术后2周内,芍药苷组大鼠进行腹腔注射20 mg/kg芍药苷,其余大鼠腹腔内注射生理盐水0.5 ml。末次注射后次日取大鼠眼球行病理切片HE染色及TUNEL凋亡检测及血红素加氧酶-1(HO-1)免疫组化检测。结果(1)眼压:造模前,3组眼压比较无统计学意义(F=1.191,P=0.319);造模后30 min,模型组及芍药苷组眼压均较之前升高,造模成功(t模型组=-25.086,t芍药苷组=-24.912,均P=0.000);造模后1周、2周,高眼压模型组与芍药苷治疗组眼压均较假手术组升高(F1周=388.430,F2周=281.504,均P=0.000);造模后,模型组与芍药苷治疗组眼压无明显不同(F=1.223,P=0.283);(2)HE染色:假手术组RGCs层细胞数量明显高于模型组及芍药苷治疗组(t模型组=7.965,t芍药苷组=4.488,P=0.000);同时,芍药苷治疗组RGCs层细胞数量高于模型组,差异具有统计学意义(t=-4.407,P=0.000);(3)TUNEL检测:假手术组未见凋亡细胞,模型组单个视野内TUNEL阳性RGCs细胞较治疗组增加,具有统计学意义(t=9.410,P=0.000);(4)免疫组化染色:假手术组HO-1蛋白弱阳性表达;模型组HO-1弱阳性表达,着色稍加深;芍药苷治疗组HO-1蛋白表达强阳性表达。3组平均光密度值差异具有统计学意义(F=6.657,P=0.004),芍药苷治疗组与模型组比较(t=-2.306,P=0.033),有统计学意义。结论芍药苷对慢性高眼压大鼠视网膜神经节细胞凋亡具有保护作用,其抑制凋亡可能是通过上调HO-1蛋白表达实现的。OBJECTIVE To investigate the effect and mechanism of paeoniflorin(PF)on retinal ganglion cells(RGCs)apoptosis in rats with chronic ocular hypertension.METHODS Thirty SD rats were randomly divided into sham operation group,intraocular hypertension model group(MG)and paeoniflorin treatment group(PTG)according to random number table method,with the right eye as the experimental eye.A rat model of chronic ocular hypertension was established by standard cauterization of superior scleral vein.In the sham operation group,only bulbar conjunctiva was cut and superficial scleral vein was untreated.Rats in paeoniflorin group were injected intraperitoneally with paeoniflorin(20 mg/kg)on the intervention day and then by daily 2 weeks after it,while the rest rats were injected intraperitoneally with 0.5 ml of normal saline.The day after the last injection,the eyeball of rats was taken for HE staining,TUNEL assay and heme oxygenase-1(HO-1)immunohistochemical detection.RESULTS(1)Intraocular pressure:Before modeling,there was no significant difference in intraocular pressure among the three groups(F=1.191,P=0.319);The intraocular pressure in the model group and paeoniflorin group increased 30 min after modeling which meant the modeling was successful(tMG=-25.086,tPTG=-24.912,all P=0.000);One week and two weeks after the model was established,the intraocular pressure in the high intraocular pressure model group and the paeoniflorin treatment group was higher than that in the sham operation group(F1week=388.43,F2week=281.504,all P=0.05).After modeling,there was no significant difference in intraocular pressure between the model group and paeoniflorin treatment group(F=1.223,P=0.283).(2)HE staining:The number of RGCs layer cells in sham operation group was significantly higher than that in model group and paeoniflorin treatment group(tMG=7.965,tPTG=4.488,all P=0.000);At the same time,the number of RGCs layer cells in paeoniflorin treatment group was higher than that in model group,and the difference was statistically significant(t=-

关 键 词：芍药苷 慢性高眼压 SD大鼠 视网膜神经节细胞 凋亡 血红素加氧酶-1 

分 类 号：R285.5[医药卫生—中药学]