加减驻景方对大鼠脉络膜新生血管模型的干预研究  

Experimental on the intervention of modified Zhujing Formula on choroidal neovascularization of rats

作　　者：陶方方[1,2] 亢泽峰 陈水龄[1] 褚文丽 刘健 周志豪[3] TAO fangfang;KANG zefeng;CHEN shuiling(Eye Hospital,China Academy of Chinese Medical Sciences,Beijing 100040,China)

机构地区：[1]中国中医科学院眼科医院,北京100040 [2]中国中医科学院西苑医院,北京100091 [3]北京市门头沟区中医院,北京102300

出　　处：《中国中医眼科杂志》2020年第8期537-543,共7页China Journal of Chinese Ophthalmology

基　　金：国家自然科学基金项目(81574032);中医药传承与创新“百千万”人才工程(岐黄工程)。

摘　　要：目的观察中药加减驻景方对实验性CNV的干预作用。方法通过659 nm氪激光诱导BN大鼠建立CNV动物模型。造模后大鼠被随机分为4组:模型组、中药组、西药组和联合组。模型组大鼠用生理盐水灌胃,中药组用加减驻景方灌胃治疗,西药组用玻璃体腔注射康柏西普治疗,联合组用玻璃体腔注射康柏西普和加减驻景方灌胃治疗。分别于光凝后7 d、14 d、21 d,进行FFA与ICGA检查。检查后每组处死6只大鼠制作病理学标本,通过HE染色观察CNV变化。结果 (1)CNV发生率:光凝后7 d、14 d、21 d,模型组与各治疗组CNV形成率均达70%以上,各组差异无统计学意义(P>0.05)。(2)FFA荧光素渗漏:模型组在光凝后14 d时最强,随后变化不明显,各治疗组FFA荧光素渗漏在光凝后7 d时最强,随后变化不明显,有荧光渗漏的光凝斑在ICGA呈充盈状态。光凝后7 d,中药组与模型组荧光素渗漏强度较高,西药组次之,联合组渗漏程度最轻,比较均有统计学意义(P<0.05)。14 d与21 d时,各治疗组荧光素渗漏强度均弱于模型组,治疗组中,中药组渗漏强度最强,西药组次之,联合组渗漏强度最弱,比较均有统计学意义(P<0.05)。(3)CNV中央厚度:随光凝后时间推移,模型组和各治疗组CNV中央厚度逐渐增加,光凝后7 d,西药组和联合组CNV中央厚度均显著低于模型组和中药组(P<0.05)。光凝后14 d与21 d时,各治疗组CNV中央厚度均低于模型组,其中,中药组CNV中央厚度最厚,西药组次之,联合组最低,比较均有统计学意义(P<0.05)。结论加减驻景方可以降低实验性CNV荧光素钠渗漏强度、抑制CNV中央厚度增加,加减驻景方联合康柏西普抑制实验性CNV生长的作用最强。OBJECTIVE To observe the effects of modified Zhujing Formula on experimental choroidal neovascularization(CNV). METHODS The CNV model was induced by 659 nm krypton laser on BN rats. After photocoagulation, the rats were randomly divided into model group, Chinese Medicine group, Western Medicine group and combination group. The model group, Chinese Medicine group and Western Medicine group were treated with normal saline, modified Zhujing Formula and intravitreal injection of Conbercept respectively, while the combination group were treated with both Chinese Medicine and Western Medicine. At the 7 th day, 14 th day and 21 st day after photocoagulation, FFA and ICGA were used to observe the changes of CNV. After FFA and ICGA, 6 rats in each group were sacrificed for pathological specimens. Changes of CNV were observed by HE staining. RESULTS From the 7 th day to 21 st day after photocoagulation, the formation rate of CNV between the model group and treatment group had no significant difference(P>0.05), which both were more than 70%. The FFA fluorescein leakage in model group was the strongest at the 14 th day after photocoagulation, and the subsequent change was not obvious. The leakage intensity of FFA was the strongest at the 7 th day after photocoagulation, and the change was not obvious in the following time in all the treatment groups. The light spot was filling in ICGA. At the 7 th day after photocoagulation, the leakage intensity of fluorescein was higher in Chinese Medicine group and the model group, followed by Western Medicine group and the combination group(P<0.05). At the 14 th day and 21 st day, the intensity of fluorescein leakage in each treatment group was weaker than that in the model group.In the treatment group, the leakage intensity of Chinese Medicine group was the strongest, followed by Western Medicine group, and the combination group was the weakest(P<0.05). With the time lapse after photocoagulation, the central thickness of CNV in model group and treatment group increased gradually. At the

关 键 词：加减驻景方 脉络膜新生血管 瞳神络病 

分 类 号：R285.5[医药卫生—中药学]