人类白细胞抗原-G在系统性红斑狼疮中的表达及对Th细胞分化的影响  

Expression of HLA-G and the effect on Th cell differentiation in systemic lupus erythematosus

作　　者：华馨 潘红 施敏 HUA Xin;PAN Hong;SHI Min(Clinical Laboratory,HwaMei Hospital,University of Chinese Academy of Science Ningbo Institute of Life and Health Industry,University of Chinese Academy of Sciences,Ningbo,Zhejiang 315010,China)

机构地区：[1]中国科学院大学宁波华美医院检验科中国科学院大学宁波生命与健康产业研究所,浙江宁波315010

出　　处：《中国卫生检验杂志》2020年第15期1848-1851,共4页Chinese Journal of Health Laboratory Technology

基　　金：宁波市公益基金资助(LY19H160011)。

摘　　要：目的检测系统性红斑狼疮(SLE)患者血清可溶性人类白细胞抗原-G(sHLA-G)水平以及外周血CD3+T细胞上HLA-G的表达水平,并分析其与Th1/Th2细胞分化的关系,探讨其在系统性红斑狼疮发病机制中的可能作用。方法选取60例SLE患者作为SLE组和60例健康体检者作为健康组,分别采用流式细胞术和酶联免疫吸附试验检测外周血CD3^+HLA-G^+T亚群的表达情况以及血清sHLA-G的表达水平;同时检测血清IFN-γ、IL-10的水平。结果SLE患者CD3^+HLA-G^+T百分比较健康组高(P<0.01);SLE患者血清sHLA-G水平显著高于健康组(P<0.01),SLE中的Th2型细胞因子IL-10显著高于健康组(P<0.01);mHLA-G和sHLA-G的变化与IL-10呈正相关(r值分别为0.507、0.706,P=0.001)(P<0.01)。结论SLE患者的CD3+HLA-G+T亚群和sHLA-G显著增加,可能参与了SLE的发病,并可作为SLE的诊断标志物。Objective To study the clinical significance of CD3+HLA-G+T cell in peripheral blood and the soluble form of HLA-G(sHLA-G)in serum with systemic lupus erythematosus(SLE),and its effect on the Th1/Th2 imbalance in systemic lupus erythematosus.Methods The study included 60 SLE patients and 60 healthy controls.Flow cytometry analysis was used to detect the percentages of CD3^+HLA-G^+T cells in peripheral blood samples,enzyme-linked immunosorbent assay was used to detect the serum concentration of sHLA-G,IFN-γand IL-10 production in SLE patients.Results The median percentages of CD3^+HLA-G^+T cells was increased in SLE than healthy control,with the difference statistically significant(P<0.01);serum sHLA-G level of SLE patients was significantly higher than that of the healthy group(P<0.01).The Th2-type cytokine,IL-10,in SLE patients was significantly higher than that of the healthy group(P<0.01).The changes of mHLA-G and sHLA-G were positively correlated with IL-10(r=0.507,r=0.706,P=0.001),and the difference was statistically significant(P<0.01).Conclusion The level of CD3+HLA-G+T cells in peripheral blood and sHLA-G in serum were significantly increase in SLE patients,which suggested that HLA-G plays roles in the lesion of SLE,and it could be used as a diagnostic marker of SLE.

关 键 词：系统性红斑狼疮 CD3^+HLA-G^+T 可溶性人类白细胞抗原-G TH1/TH2 

分 类 号：R593.241[医药卫生—内科学]