慢性粒细胞白血病中ID1表达的临床意义及其作用机制  

Clinical significance and mechanism of ID1 expression in chronic myeloid leukemia

作　　者：马吉春[1] 李新雄 唐丽娟 周静东[3] 袁畅 钱军[3] 林江[1] MA Jichun;LI Xinxiong;TANG Lijuan;ZHOU Jingdong;YUAN Chang;QIAN Jun;LIN Jiang(Central Laboratory,the Affiliated People′s Hospital of Jiangsu University,Zhenjiang,Jiangsu 212002,China;Jiangsu University,Zhenjiang,Jiangsu 212002,China;Department of Hematology,the Affiliated People′s Hospital of Jiangsu University,Zhenjiang,Jiangsu 212002,China)

机构地区：[1]江苏大学附属人民医院中心实验室,江苏镇江212002 [2]江苏大学,江苏镇江212002 [3]江苏大学附属人民医院血液科,江苏镇江212002

出　　处：《现代医药卫生》2020年第18期2860-2863,共4页Journal of Modern Medicine & Health

基　　金：国家自然科学基金项目(81900163、81900166、81970118);中国博士后科学基金第60批面上项目(2016M601748);江苏省卫生健康委员会高层次卫生人才“六个一工程”拔尖人才项目(LGY2018024);江苏省卫生健康委员会“科教强卫”工程青年医学人才项目(QNRC2016450);江苏省镇江市科学技术局重点研发计划(社会发展)项目(SH2018044);江苏大学学生科研立项项目(18AD0153)。

摘　　要：目的通过分析分化抑制因子-1(ID1)在慢性粒细胞白血病(CML)中的表达及其临床意义,揭示ID1促进CML发生、发展的作用及其机制。方法选取2016年10月至2018年4月江苏大学附属人民医院门诊及住院共35例CML患者(CML组)及19例健康供髓者(健康对照组)为研究对象。采用实时荧光定量聚合酶链式反应方法检测两组骨髓单个核细胞中ID1的表达水平;基因表达综合(GEO)数据库(GSE4170)分析CML不同临床分期ID1表达情况;CCK8试剂、流式细胞术检测ID1对白血病K562细胞增殖和凋亡的影响。结果健康对照组中ID1的中位表达水平为0.0371,CML组中ID1的中位表达水平为0.2127,ID1在CML患者中表达上调(P<0.0001)。在GEO数据库(GSE4170)的CML不同临床分期资料中,23例慢性期(CP期)、15例加速期(AP期)和36例急变期(BC期)患者ID1的中位表达水平分别为-0.0053、0.2112和0.3416;AP期和BC期ID1患者表达水平均显著高于CP期,差异均有统计学意义(P<0.01)。与对照组的K562(K562-NC)细胞相比,过表达ID1的K562(K562-ID1)细胞增殖增加,差异有统计学意义(P<0.05或0.01)。K562-ID1细胞的总体凋亡率低于K562-NC细胞,差异有统计学意义(P<0.01)。结论ID1在CML患者中高表达,可能通过促进细胞增殖、抑制凋亡参与CML的进展。Objective To analyze expression of differentiation inhibitor-1(ID1)in chronic myeloid leukemia(CML)and its clinical significance.And to reveal the role and mechanism of ID1 in occurrence and development of CML.Methods A total of 35 CML patients(CML group)and 19 healthy marrow donors(healthy control group)were selected from October 2016 to April 2018 admitted to the Outpatient and Inpatient Departments of the Affiliated People′s Hospital of Jiangsu University.Real-time quantitative polymerase chain reaction method was used to detect the expression level of ID1 gene in two groups of bone marrow mononuclear cells;gene expression comprehensive(GEO)database(GSE4170)was used to analyze the expression of ID1 in different clinical stages of CML;effect of ID1 on proliferation and apoptosis were analyzed by CCK8 and flow cytometry in leukemia K562 cells.Results The median expression level of ID1 in the healthy control group was 0.0371,and the median expression level of ID1 in the CML group was 0.2127;ID1 expression was up-regulated in CML patients(P<0.0001).In the different clinical stages date of CML in GEO database(GSE4170),the median expression levels of ID1 in 23 patients with CP stage,15 patients with AP stage and 36 patients with BC stage were-0.0053,0.2112 and 0.3416 respectively;ID1 expression levels in AP stage and BC stage were significant higher than that in CP stage,the differences were statistically significant(P<0.01).Compared with K562 cells in the control group(K562-NC),the proliferation of K562 cells overexpressing ID1 increased(K562-ID1),and the difference was statistically significant(P<0.05 or 0.01).The overall apoptosis rate of K562-ID1 cells was lower than that of K562-NC cells,and the difference was statistically significant(P<0.01).Conclusion ID1 is highly expressed in CML patients and may participate in the progression of CML by promoting the proliferation of cells and inhibiting apoptosis.

关 键 词：分化抑制因子-1 慢性髓系白血病 临床意义 机制研究 K562细胞 

分 类 号：R557[医药卫生—血液循环系统疾病]