滴鼻感染甲型流感病毒对BALB/c和C57BL/6J小鼠自发行为学的比较研究  

Comparison on spontaneous behavior of BALB/c and C57BL/6J mice infected intranasally with influenza A virus

作　　者：周艳琳 谷李铭 李康生[2] 王革非(指导)[2] ZHOU Yan-Lin;GU Li-Ming;LI Kang-Sheng;WANG Ge-Fei(Department of Pathogen Biology and Immunology,Sanquan College of Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院三全学院病原生物学与免疫学教研室,新乡453003 [2]汕头大学医学院微生物学与免疫学教研室,广东省感染病与分子免疫病理重点实验室,汕头515041

出　　处：《中国免疫学杂志》2020年第16期1931-1934,1940,共5页Chinese Journal of Immunology

基　　金：河南省科技攻关项目(192102310333);河南省医学科技攻关计划联合共建项目(2018020385);新乡医学院三全学院骨干教师培育项目(SQ2020GGJS01)资助。

摘　　要：目的:比较滴鼻感染甲型流感病毒PR8的C57BL/6J和BALB/c两种品系小鼠旷场自发行为表现的差异,探讨流感病毒感染对两种品系小鼠抑郁焦虑情绪的影响。方法:健康C57BL/6J小鼠和BALB/c小鼠各20只,随机分为甲型流感病毒PR8感染组(PR8组)和生理盐水对照组(CON组),每组10只。记录每组小鼠1周内的体质量、在旷场行为箱体内的行走总路程、周边区域运动路程、平均运动速度及粪便排泄情况,并检测感染后的第7天小鼠脑组织炎症因子(TNF-α、IL-1β、CXCL10、BDNF)转录水平。结果:滴鼻感染PR8后均引起小鼠体质量显著下降,且BALB/c小鼠较C57BL/6J小鼠体质量下降更明显。BALB/c小鼠较C57BL/6J小鼠在旷场试验中行走总路程、周边区域运动路程减少更明显。感染后第7天BALB/c小鼠中枢神经系统(CNS)炎症因子表达显著上调。结论:滴鼻感染PR8可引起小鼠体质量下降,活动性降低,出现一定程度的抑郁焦虑情绪,并伴随CNS炎症因子显著上调。且BALB/c小鼠对PR8的敏感度较C57BL/6J小鼠明显,更适宜做PR8感染的模型小鼠。本研究为研究流感病毒外周感染诱导小鼠空间记忆、学习认知、情绪等调控奠定基础,为进一步探究流感相关脑病的病理机制提供依据。Objective:To compare the difference of spontaneous behavior in open field of C57BL/6J mice and BALB/c mice infected intranasally with influenza A virus PR8 and explore the effect on depression and anxiety of two strains of mice infected with influenza virus.Methods:20 healthy C57BL/6J mice and 20 healthy BALB/c mice were randomly divided into influenza A PR8 infection group(PR8 group)and normal saline control group(CON group),10 mice in each group.We recorded body weight of mice in each group within a week,furthermore we observed and recorded the total walking distance in open-field behavior box,the movement distance in surrounding area,average speed and the excretion of feces of every one mouse and detected the transcription levels of inflammatory factors(TNF-α,IL-1β,CXCL10,BDNF)in brain of mice on 7th day.Results:Mice following influenza A virus PR8 infection bodyweight declined sharply,and the weight loss in BALB/c mice was more significant than that in C57BL/6J mice.BALB/c mice infected with PR8 had a more significant decrease in total walking distance and surrounding area distance than that in C57BL/6J mice in open field experiment.Expressions of inflammatory factors were significantly up-regulated in CNS of BALB/c mice on day 7.Conclusion:Mice infected with influenza A virus PR8 can lead to declined body weight,decreased activity,and a certain extent of depression and anxiety,moreover the inflammatory factors were accompanied by significant up-regulation of mice in CNS.In addition,BALB/c mice were more sensitive to PR8 than C57BL/6J mice.It indicated BALB/c mice maybe more suitable to be the model mice infected with PR8.This study has a great significance to investigate the regulation of spatial memory,learning,cognition and emotion of mice induced by peripheral infection of influenza virus and laid a foundation for further exploring the pathological mechanism of influenza related encephalopathy.

关 键 词：流感病毒 小鼠 旷场自发行为 抑郁情绪 

分 类 号：R373.1[医药卫生—病原生物学]