中药大黄对重症急性胰腺炎肺损伤大鼠肺泡巨噬细胞凋亡的影响  

Effect of Chinese medicine Rhubarb on apoptosis of alveolar macrophages in rats with severe acute pancreatitis and lung injury

作　　者：李孝全 莫静欣 LI Xiao-Quan;MO Jing-Xin(Department of Emergency&Critical Care,Remin Hospital of Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院附属人民医院急危重症中心,十堰442000

出　　处：《中国免疫学杂志》2020年第15期1839-1843,共5页Chinese Journal of Immunology

摘　　要：目的:研究中药大黄对重症急性胰腺炎(SAP)大鼠肺泡巨噬细胞凋亡影响并探讨其作用机制。方法:牛磺胆酸钠建立大鼠SAP模型;流式细胞术检测大黄对SAP大鼠肺泡巨噬细胞损伤的影响;ELISA法检测大黄治疗的SAP大鼠肺泡巨噬细胞中TNF-α、NO含量;qRT-PCR法检测大黄治疗的SAP大鼠肺泡巨噬细胞中TNF-α、iNOS mRNA水平;Western blot检测大黄治疗的SAP大鼠肺泡巨噬细胞中p-JAK2、p-STAT3蛋白表达。结果:成功建立大鼠SAP模型;大黄治疗后,模型大鼠肺泡巨噬细胞中TNF-α、NO含量、TNF-α、iNOS mRNA表达及p-JAK2、p-STAT3蛋白表达均显著降低(P<0.05)。结论:中药大黄可促进SAP大鼠肺泡巨噬细胞凋亡,其机制可能与失活JAK2/STAT3信号通路、抑制炎症因子分泌有关,可能为大黄的临床应用提供依据。Objective:To study effect of Chinese medicine Rhubarb on apoptosis of alveolar macrophages in rats with severe acute pancreatitis(SAP),and to explore its mechanism.Methods:Rat model of SAP was established by using sodium taurocholate.Flow cytometry was used to detect effect of Rhubarb on alveolar macrophage injury in rats with SAP.ELISA was used to detect TNF-αand NO on alveolar macrophage in rats with SAP treated with rhubarb.Levels of TNF-αand iNOs in phagocytes were detected by qRT-PCR.Protein expression of p-JAK2 and p-STAT3 in alveolar macrophages of rate with SAP were detected by Western blot.Results:Model of SAP in rats was successfully established.After treatment with rhubarb,the content of TNF-αand NO in alveolar macrophages of model rats was significantly decreased,TNF-α,NO,mRNA of TNF-αand iNOS and expression of p-JAK2,p-STAT3 protein were significantly reduced(P<0.05).Conclusion:Chinese medicine Rhubarb can promote apoptosis of alveolar macrophages in rats with SAP.The mechanism may be related to inactivation of JAK2/STAT3 signaling pathway and inhibition of inflammatory factor secretion,which may provide a basis for clinical application of rhubarb.

关 键 词：大黄 重症急性胰腺炎 凋亡 JAK2/STAT3信号通路 炎症因子 

分 类 号：R563[医药卫生—呼吸系统] R576[医药卫生—内科学]