两种助剂对溴敌隆毒饵的增效作用研究  

Study on the Synergism of Two Adjuvants on Bromadiolone Baits

作　　者：姜洪雪 姚丹丹[1] 冯志勇[1] JIANG Hongxue;YAO Dandan;FENG Zhiyong(Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室,广东广州510640

出　　处：《广东农业科学》2020年第8期103-109,共7页Guangdong Agricultural Sciences

基　　金：广东省现代农业产业技术体系创新团队项目(2019KJ113);广东省农业科学院院长基金(201932);广东省农业科学院青年导师制项目(R2018QD-063)。

摘　　要：【目的】为提高杀鼠剂的利用率及防控效果,降低其生态风险,研究了渗透剂对抗凝血灭鼠剂溴敌隆的增效作用。【方法】采用高效液相色谱法,检测添加不同种类及不同浓度的助剂后稻谷毒饵中溴敌隆的含量分布;采用有选择摄食试验和无选择摄食试验的生物测定方法,分别测试不同方法制成的溴敌隆毒谷对小鼠的适口性及毒杀效果。【结果】经4%N,N-二甲基甲酰胺及4%二甲基亚砜处理后,0.01%溴敌隆毒谷糙米中的药物含量均显著提高,比常规的0.01%溴敌隆毒谷分别增加271.17%和163.51%;而添加4%N,N-二甲基甲酰胺和4%二甲基亚砜配制的0.0025%溴敌隆毒谷,糙米中的药物含量均显著高于常规0.0025%溴敌隆毒谷,分别增加433.33%和400.95%;生物测定结果表明,用4%的两种助剂配制的0.0025%溴敌隆毒谷,对小白鼠均具有较好的适口性及毒杀效果,摄食系数分别为2.69和0.80;毒杀率分别为100%和90%,均显著高于常规的0.0025%溴敌隆毒谷,与0.01%溴敌隆毒谷无显著差异。【结论】使用4%N,N-二甲基甲酰胺或4%二甲基亚砜作为助剂对溴敌隆毒饵均有显著的增效作用,并可显著降低杀鼠剂的使用浓度。【Objective】In order to increase the utilization rates and prevention and control effects of anticoagulant rodenticides and reduce their ecological risks,the synergistic effects of two adjuvants on bromadiolone baits were investigated.【Method】The content distribution of bromadiolone in the poison grains prepared by different varieties and concentrations of adjuvants were determined by using high-performance liquid chromatography.The palatability and the toxic effects of the bromodiolone poison grains prepared with different adjuvants for mice were evaluated by selective and non-selective feeding tests.【Result】The content of bromadiolone in the brown rice of 0.01% poison grains treated with 4% N,N-dimethylformamide and 4% dimethyl sulfoxide was significantly higher than that in the 0.01% poison grains,with the increase of 271.17% and 163.51%,respectively.While the content of bromadiolone in the brown rice of 0.0025% poison grains treated with 4% N,N-dimethylformamide and 4% dimethyl sulfoxide was significantly higher than that in the 0.0025% poison grains,with the increase of 433.33% and 400.95%,respectively.The results of bioassay showed that the 0.0025% bromodiolone poison grains with two kinds of adjuvants exhibited good palatability and potent efficacy on mice.Their feeding coefficient were 2.69 and 0.80,with the poison rates of 100% and 90%,which were significantly higher than the poison rate of the 0.0025% bromodiolone poison grains,but there was no significant difference with the 0.01% bromodiolone poison grains.【Conclusion】4% N,N-dimethylformamide and 4% dimethyl sulfoxide showed significant synergistic effects to the bromodiolone poison baits,and decreased the use concentration of rodenticide obviously.

关 键 词：N N-二甲基甲酰胺 二甲基亚砜 溴敌隆 毒饵 小白鼠 摄食系数 毒杀效果 

分 类 号：S482.92[农业科学—农药学] S482.5[农业科学—植物保护]