干热法灭活对新型冠状病毒肺炎相关炎症指标检测的影响  

Influence of dry heat inactive treatment for the determination of inflammatory indicators related with corona virus disease 2019

作　　者：赵明海[1] 李欣[1] 魏东[1] 罗敏[1] 张雅洁[1] 杨懿祺 郑磊[1] ZHAO Minghai;LI Xin;WEI Dong;LUO Min;ZHANG Yajie;YANG Yiqi;ZHENG Lei(Department of Clinical Laboratory,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong,China)

机构地区：[1]南方医科大学南方医院检验科,广东广州510515

出　　处：《检验医学》2020年第9期924-927,共4页Laboratory Medicine

摘　　要：目的探讨血样本经56℃30 min干热灭活后,对新型冠状病毒肺炎(COVID-19)诊疗相关炎症因子检测结果的影响程度。方法收集进行过C反应蛋白(CRP)、降钙素原(PCT)及白细胞介素6(IL-6)检测,且结果尽量覆盖线性范围的临床剩余血样本332例。所有样本于收集当天进行56℃30 min干热灭活,比较灭活前、后检测结果的差异。结果CRP灭活前、后检测结果差异无统计学意义(P=0.167),IL-6、PCT灭活前、后检测结果差异有统计学意义(P值分别为0.002和<0.001)。有98例(79.03%)样本灭活后PCT水平下降。灭活前、后3个炎症指标结果均有较好的一致性,PCT、CRP及IL-6分别有96.77%、97.11%及97.11%的散点位于差值的95%可信区间,相关系数(r值)分别为0.992、0.999和0.996。结论56℃30 min干热灭活对CRP的检测结果无影响,对PCT、IL-6的检测结果有影响。建议实验室关注COVID-19患者样本传染性风险的前处理流程。Objective To investigate the influence of dry heat inactive treatment(56℃30 min)for the determination of inflammatory indicators related with corona virus disease 2019(COVID-19)diagnosis and treatment.Methods Totally,332 clinical residual blood samples which C-reactive protein(CRP),procalcitonin(PCT)and interleukin 6(IL-6)determinations were carried out were collected.All the samples were inactivated by dry heat inactive treatment at 56℃for 30 min on the day of collection,and the difference of results was compared.Results There was no statistical significance in CRP determination results before and after inactivation(P=0.167),and there was statistical significance in IL-6 and PCT determination results before and after inactivation(P=0.002,P<0.001),of which the PCT determination results of 98(79.03%)samples decreased after inactivation.The determination results of the 3 inflammatory indicators before and after inactivation had good consistency,and PCT,CRP and IL-6 had 96.77%,97.11%and 97.11%scatter points in 95%confidence intervals,respectively.The correlation coefficients(r)were 0.992,0.999 and 0.996,respectively.Conclusions Dry heat inactive treatment at 56℃for 30 min has no effect on CRP determination results,but has effect on PCT and IL-6 determination results.It should pay attention to the pretreatment process of dry heat inactive treatment,in order to reduce the infectious risk of COVID-19 patients'samples.

关 键 词：新型冠状病毒肺炎 干热法灭活 炎症指标 C反应蛋白 降钙素原 白细胞介素6 

分 类 号：R446.1[医药卫生—诊断学]