载脂蛋白M在非小细胞肺癌A549细胞增殖、迁移和侵袭中的作用  

Role of apolipoprotein M in the proliferation,migration and invasion of non-small cell lung cancer A549 cells

作　　者：王子谨 张晓膺[1] WANG Zijin;ZHANG Xiaoying(Department of Cardiothoracic Surgery,the Third Affiliated Hospital of Soochow University,Changzhou 213003,Jiangsu,China)

机构地区：[1]苏州大学附属第三医学院心胸外科,江苏常州213003

出　　处：《检验医学》2020年第9期937-942,共6页Laboratory Medicine

摘　　要：目的探讨载脂蛋白M(apo M)对非小细胞肺癌(NSCLC)A549细胞系增殖、迁移、侵袭的影响及与基质金属蛋白酶(MMP)-10的关系。方法以转染apo M慢病毒的A549细胞作为实验组(apo M-OE组),转染空慢病毒的A549细胞作为阴性对照组。分别采用CCK-8法、划痕实验和Transwell侵袭实验检测各组细胞的增殖、迁移和侵袭能力。采用实时荧光定量聚合酶链反应(qRT-PCR)和免疫印迹法检测各组细胞apo M及MMP-10的表达水平。结果与阴性对照组相比,apo M-OE组apo M表达明显上调(P<0.01),表达量约为阴性对照组的28倍。apo M-OE组细胞增殖倍数及侵袭率明显高于阴性对照组(P<0.001)。与阴性对照组比较,apo M-OE组空白视野显著减少,闭合速率显著加快,即细胞迁移率更大(P<0.001)。apo M-OE组中MMP-10 mRNA及apo M、MMP-10蛋白表达量显著高于阴性对照组(P<0.05)。结论过表达apo M可增强A549细胞的增殖、迁移和侵袭能力,同时使细胞高表达MMP-10。Objective To investigate the influence of apolipoprotein M(apo M)on the proliferation,migration and invasion of non-small cell lung cancer(NSCLC)A549 cell line and its relationship with matrix metalloproteinase(MMP)-10.Methods A549 cells being transfected with apo M lentivirus were used as apo M-OE group,and those being transfected with A549 empty lentivirus were used as negative control group.CCK-8 method,scratch test and Transwell invasion test were used to determine the proliferation,migration and invasion abilities.The expression levels of apo M and MMP-10 were determined by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)and immunoblotting.Results Compared with negative control group,the expression level of apo M in apo M-OE group was up-regulated(P<0.01),and the expression level was about 28 times higher than that of negative control group.The proliferation and invasion rates of apo M-OE group were higher than those of negative control group(P<0.001).Compared with negative control group,the blank field of vision in apo M-OE group was reduced,the closing rate was accelerated,and the migration rate was greater(P<0.001).The expression levels of MMP-10 mRNA,apo M protein and MMP-10 protein in apo M-OE group were higher than those in negative control group(P<0.05).Conclusions Over-expressing apo M can promote the proliferation,migration and invasion of A549 cells,and can make the cells highly expressing MMP-10.

关 键 词：载脂蛋白M 基质金属蛋白酶-10 非小细胞肺癌 

分 类 号：R446.1[医药卫生—诊断学]