柔嫩艾美耳球虫感染对罗曼粉鸡盲肠组织病理学和细胞免疫相关基因的影响  

Effect of Eimeria tenella Infection on the Histopathology and Cellular Immunity-related Gene Expression in Cecum of Roman Chicken

作　　者：闫志强[1] 付文贵[1] 陈春林[1] 唐红梅[1] 郑华[1] 朱买勋[1] 翟少钦[1] YAN Zhiqiang;FU Wengui;CHEN Chunlin;TANG Hongmei;ZHENG Hua;ZHU Maixun;ZHAI Shaoqin(Chongqing Academy of Animal Sciences,Chongqing 402460,China)

机构地区：[1]重庆市畜牧科学院,重庆402460

出　　处：《云南农业大学学报（自然科学版）》2020年第5期789-795,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：重庆市自然科学基金项目(cstc2019jcyj-msxmX0061);重庆市现代山地特色高效农业技术体系(2018[5]号)。

摘　　要：【目的】明确柔嫩艾美耳球虫感染对罗曼粉鸡盲肠组织病理学和细胞免疫相关基因mRNA表达量的影响。【方法】将100只鸡随机分为空白组、感染Ⅰ、Ⅱ、Ⅲ和Ⅳ组,每组20只,感染Ⅰ、Ⅱ、Ⅲ和Ⅳ组分别口服3×104、2×104、1×104和0.5×104个孢子化鸡柔嫩艾美耳球虫卵囊,空白组口服相同体积的生理盐水。试验结束时,剖解试验鸡,采集盲肠制作病理切片,观察组织病理学变化并进行评分;采用荧光定量PCR方法检测盲肠IL-2、IL-17、TGF-β1和IFN-γ基因mRNA表达量。【结果】感染Ⅰ、Ⅱ和Ⅲ组试验鸡黏膜层破坏严重,出血明显,大量炎性细胞浸润,腺上皮细胞内见大量虫体,病理损伤评分为重度损伤;感染柔嫩艾美耳球虫第5、7天,感染组盲肠组织IL-2、IL-17、TGF-β1和IFN-γ基因mRNA表达量均升高,与空白组相比差异极显著(P<0.01)。【结论】不同剂量的柔嫩艾美耳球虫可造成鸡盲肠组织不同程度损伤,且能上调盲肠组织IL-2、IL-17、TGF-β1和IFN-γ基因的表达。[Purpose]To determine the effect of Eimeria tenella infection on the histopathology and mRNA expression of cellular immunity related genes in Roman powder chickens.[Method]100 chickens were randomly divided into blank control group,infected group I,II,III and IV,20 in each group,and infected group I,II,III and IV were orally administered 3×104,2×104,1×104 and 0.5×104 spores of chicken E.tenella oocysts,the blank control group orally the same volume of normal saline,at the end of the test,dissection test chicken,collecting cecum to make pathological sections,observe histopathological changes and score.At the same time,the mRNA expression levels of IL-2,IL-17,TGF-β1 and IFN-γgenes in the cecum were detected by real-time PCR.[Results]In the infected group I, II and III, the mucosal layer of the test group was severely damaged and the bleeding was obvious.A large number of inflammatory cells infiltrated, and a large number of worms were found inthe glandular epithelial cells. The pathological damage score was severely injured;after infection withE. tenella. The expression of IL-2, IL-17, TGF-β1 and IFN-γ mRNA in the cecal tissue of the infectedgroup increased at the 5th and 7th days and the difference was significant compared with the blankgroup (P<0.01).[Conclusion]Different doses of E. tenella can cause different degrees of damageto chicken cecal tissue, and can induce the expression of IL-2, IL-17, TGF-β1 and IFN-γ genes incecaltissue.

关 键 词：柔嫩艾美耳球虫 盲肠 组织病理学 基因表达 

分 类 号：S852.7[农业科学—基础兽医学]