吉马酮激活AMPK诱导宫颈癌Hela细胞自噬和凋亡  

Gemmarone activates AMPK to induce autophagy and apoptosis of in cervical cancer Hela cells

作　　者：姚宗花[1] 张先娟[1] 郭敬敬 刘美艳 张丽丽 YAO Zong-Hua;ZHANG Xian-Juan;GUO Jing-Jing;LIU Mei-Yan;ZHANG Li-Li(Department of Gynecology,Binzhou Medical College Affiliated Hospital,Binzhou 256603,China)

机构地区：[1]滨州医学院附属医院妇产科,滨州256603 [2]临沂市妇女儿童医院妇产科,临沂276000

出　　处：《中国免疫学杂志》2020年第17期2105-2108,2114,共5页Chinese Journal of Immunology

基　　金：山东省自然科学基金(No.ZR2018LH017)资助。

摘　　要：目的:探讨天然化合物吉马酮(GMC)对人宫颈癌Hela细胞自噬及凋亡的影响。方法:CCK-8法检测GMC对宫颈癌Hela细胞增殖的影响;流式细胞仪检测细胞凋亡,Western blot检测细胞自噬相关蛋白表达水平,免疫荧光检测LC3含量。结果:GMC明显抑制宫颈癌Hela细胞增殖,诱导宫颈癌Hela细胞凋亡,且呈浓度依赖性。GMC可促进宫颈癌Hela细胞自噬,LC3含量随GMC浓度增加而升高,且AMP依赖蛋白激酶(AMPK)水平明显升高(P<0.01)。结论:GMC可通过激活AMPK信号通路抑制Hela细胞增殖,诱导其凋亡和自噬。Objective:To investigate effect of natural compound gemmarone(GMC)on autophagy and apoptosis of human cervical cancer Hela cells.Methods:CCK-8 assay was used to detect effect of GMC on Hela cell proliferation in cervical cancer.Flow cytometry was used to detect apoptosis.Western blot was used to detect expression levels of autophagy related proteins,and immunofluorescence was used to detect content of LC3.Results:GMC significantly inhibited proliferation of cervical cancer Hela cells and induced apoptosis of cervical cancer Hela cells in dependent concentration.GMC promoted autophagy in cervical cancer Hela cells,LC3 content increased along with concentration increasing of GMC,and AMPK level significantly increased(P<0.01).Conclusion:GMC can inhibit Hela cell proliferation and induce apoptosis and autophagy by activating AMPK signaling pathway.

关 键 词：吉马酮 宫颈癌 细胞凋亡 自噬 

分 类 号：R392[医药卫生—免疫学]