双光子受激发射损耗复合显微成像技术研究  

Two-photon stimulated emission depletion composite microscopy

作　　者：魏通达 张运海[1] 昌剑[1] 季林[1] 杨皓旻[1] 缪佳 WEI Tongda;ZHANG Yunhai;CHANG Jian;JI Lin;YANG Haomin;MIAO Jia(Key Laboratory of medical optics,Suzhou Institute of biomedical engineering and technology,Chinese Academy of Sciences,Suzhou 215163,China;Suzhou Branch,Jiangsu Institute of Medical Devices Testing,Suzhou 215163,China)

机构地区：[1]中国科学院苏州生物医学工程技术研究所医用光学重点实验室,江苏苏州215163 [2]江苏省医疗器械检验所苏州分所,江苏苏州215163

出　　处：《光学仪器》2020年第4期67-74,共8页Optical Instruments

基　　金：国家重点研发计划(2017YFC0110305);江苏省自然科学基金(BK20170390);江苏省博士后基金(2018K114C)。

摘　　要：鉴于双光子受激发射损耗(STED)复合显微镜在神经疾病临床诊断及脑科学研究中的重要作用,对双光子STED复合显微成像中多波长选通、多光束合束、关键技术指标等进行了研究,完成了复合显微镜样机系统集成研制和复合成像。该复合显微镜可以对荧光标记的样本进行扫描成像,具备红绿双色荧光扫描成像功能、双光子绿色荧光成像功能和STED超分辨绿色荧光成像功能。测试结果表明,该复合显微镜成像深度达到700μm,分辨率优于60 nm。Two-photon(2P)stimulated emission depletion(STED)composite microscope has the potential to be widely used in clinical diagnosis of neurological diseases and brain science research.In this paper,based on the research of multi wavelengths selection,multi beams combination,key technical indexes testing research,the integrated development of composite microscopy prototype system and composite imaging are completed.The composite microscope can image the fluorescent labeled samples,which has the functions of red-green two-color fluorescence imaging,2P green fluorescence imaging and STED super-resolution green fluorescence imaging.In terms of index,the imaging depth is 700μm,and the resolution of STED imaging is better than 60 nm.

关 键 词：荧光显微 双光子显微 超高分辨率 受激发射损耗 扫描显微 

分 类 号：O436[机械工程—光学工程]