白术多糖对小鼠淋巴细胞的免疫调节作用  

Immunomodulatory effect of Atractylodis macrocephalae Koidz.polysaccharides on mouse lymphocytes

作　　者：徐伟[1] 方思佳 关然[1] 张岑容 胡松华[1] XU Wei;FANG Si-Jia;GUAN Ran;ZHANG Cen-Rong;HU Song-Hua(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学动物科学学院,杭州310058 [2]磐安县农业局,金华322300

出　　处：《中国免疫学杂志》2020年第13期1573-1577,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金青年基金(31802233);中国博士后科学基金面上项目(2018M642456);兽医学(中医药)北京市重点实验室开放课题(TCVM-201903)资助。

摘　　要：目的:探讨白术多糖对小鼠脾淋巴细胞的免疫调节作用。方法:将白术多糖和ConA或LPS及小鼠脾淋巴细胞在体外共培养,MTT法检测淋巴细胞转化;ELISA法检测细胞培养上清中细胞因子(IL-2、IL-6、IL-10和TNF-α)和IgG含量;RT-qPCR法检测白术多糖联合ConA或LPS对转录因子T-bet、Gata3和NF-κB mRNA表达的影响;流式细胞术检测白术多糖联合LPS对CD3、CD4、CD8和CD19淋巴细胞亚群比例的影响。结果:白术多糖促进ConA诱导的脾脏T淋巴细胞转化、IL-2、IL-6、IL-10和TNF-α分泌和转录因子(T-bet和Gata3)mRNA表达,抑制LPS对脾脏B淋巴细胞的激活,减少TNF-α和IgG分泌,降低NF-κB mRNA表达水平,降低LPS诱导的CD3、CD4和CD8淋巴细胞亚群比例。结论:白术多糖可促进ConA诱导的淋巴细胞转化,但抑制LPS诱导的淋巴细胞转化。Objective:To investigate immunomodulatory effect of RAMPtp on mouse splenic lymphocytes.Methods:Splenocytes were co-cultrued with RAMPtp in presence of ConA or LPS and then determined for lymphocyte proliferation by MTT.ELISA was employed to detect contents of cytokines(IL-2,IL-6,IL-10 and TNF-α)and IgG in supernatants.RT-qPCR was performed to measure T-bet,Gata3 and NF-κB mRNA expressions in splenocytes stimulated by RAMPtp combined with ConA or LPS.Flow cytometry was conducted to measure effect of RAMPtp on percentages of lymphocyte subpopulation of CD3,CD4,CD8 and CD19 induced by LPS.Results:RAMPtp promoted proliferation of ConA-induced splenic T lymphocytes,secretions of IL-2,IL-6,IL-10 and TNF-α,and mRNA expressions of transcription factors(T-bet and Gata3),and inhibited activation of splenic B lymphocytes stimulated by LPS,decreased secretions of TNF-αand IgG,and reduced mRNA level of NF-κB,and decreased CD3,CD4 and CD8 subpopulation induced by LPS.Conclusion:RAMPtp can promote lymphocyte proliferative response to ConA,but inhibit response to LPS.

关 键 词：白术多糖 淋巴细胞 免疫调节 细胞因子 转录因子 

分 类 号：R967[医药卫生—药理学]