不同抗性苹果品种应答轮纹病菌胁迫的差异蛋白质组分析  

Proteome Analysis of Different Resistant Apple Cultivars in Response to the Stress of Ring Rot Disease

作　　者：张彩霞[1] 袁高鹏 韩晓蕾[1] 李武兴[1] 丛佩华[1] Caixia Zhang;Gaopeng Yuan;Xiaolei Han;Wuxing Li;Peihua Cong(Key Laboratory of Horticulture Crops Germplasm Resources Utilization,Ministry of Agriculture,Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng 125100,China)

机构地区：[1]中国农业科学院果树研究所,农业部园艺作物种质资源利用重点实验室,兴城125100

出　　处：《植物学报》2020年第4期430-441,共12页Chinese Bulletin of Botany

基　　金：中央级公益性科研院所基本科研业务费专项(No.Y2019XK09);农业部现代农业产业技术体系建设专项资金(No.CARS-27);中国农业科学院科技创新工程(No.CAAS-ASTIP-2016-RIP-02)。

摘　　要：为鉴定不同抗性苹果(Malusdomestica)品种响应轮纹病菌胁迫的抗性相关蛋白表达差异,以抗病品种华月及易感品种金冠为试材,采用高通量同位素标记定量(IBT)技术结合液相色谱-串联质谱(LC-MS)鉴定技术,对病原菌处理前后抗、感病品种叶片的蛋白质组差异表达进行分析,共鉴定出171个差异表达蛋白(DEPs)。GO富集及KEGG通路分析表明,在细胞组分、分子功能和生物过程3类中共注释到686个GO条目,其中52个DEPs注释于KEGG通路的18个显著差异途径(P<0.05)。亚细胞定位预测分析表明,171个DEPs中有170个分别定位于8类细胞器。蛋白功能注释分析表明,46个DEPs注释于7类抗性相关蛋白,包括类甜蛋白、过氧化物酶、多酚氧化酶、过敏原蛋白、几丁质酶、内切葡聚糖酶以及主乳胶蛋白。此外,还对抗性相关蛋白的表达特点及基因定量结果进行了分析。该研究结果可为进一步解析抗、感病苹果品种应答轮纹病菌胁迫的抗性机制提供参考。The aim of this study was to investigate the differential expression of resistance related proteins in resistant and susceptible apple leaves in response to ring rot disease.The resistant and susceptible apple cultivars Huayue and Golden Delicious were analyzed by high-throughput isobaric tag(IBT)marker quantitative analysis combined with liquid chromatography-tandem mass spectrometry(LC-MS),proteome differential expression of resistant and susceptible apple leaves before and after pathogen treatment was analyzed,and 171 differential expression proteins(DEPs)were identified.GO enrichment and KEGG pathway analysis showed that 686 GO entries were annotated in cell components,molecular functions,and biological processes,of which 52 DEPs were annotated in 18 distinct pathways of KEGG pathway(P<0.05).Subcellular localization prediction analysis showed that 170 DEPs in 171 DEPs were located in 8 organelles.Functional annotation analysis showed that 46 DEPs were annotated in 7 classes of resistance-related proteins,including the thaumatin-like proteins,peroxidases,polyphenol oxidases,Mal d1 proteins,chitinases,endo-1,3-beta-glucosidases and MLP-like proteins.In addition,the expression characteristics and gene quantitative results of resistance-related proteins were analyzed.The results of this study provided a technical reference for further understanding the resistance mechanism of resistant and susceptible apples to the stress of ring rot disease.

关 键 词：苹果 抗、感病 IBT定量蛋白质组学 轮纹病 抗性相关蛋白 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]