植物转录因子与DNA互作研究技术  

Methods for Examining Transcription Factor-DNA Interaction in Plants

作　　者：杨立文 刘双荣 李玉红 林荣呈[1] Liwen Yang;Shuangrong Liu;Yuhong Li;Rongcheng Lin(Key Laboratory of Photobiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China)

机构地区：[1]中国科学院植物研究所光生物学重点实验室,北京100093

出　　处：《植物学报》2020年第4期468-474,共7页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.31870213)。

摘　　要：转录通过调控下游基因的时空特异性表达影响植物生长发育。在转录调控机制的解析过程中,转录因子与DNA的相互作用是关键的一环。近年来,研究者利用酵母单杂交(Y1H)和凝胶阻滞迁移率实验(EMSA)检测转录因子能否直接结合DNA;而瞬时表达技术则是一种检测转录因子对下游基因调控作用的便捷方式。该文对Y1H、EMSA和瞬时表达技术的原理、实验方法和相关注意事项进行详细阐述,以期为转录因子与DNA的互作研究提供参考方法。Transcription affects the growth and development of plants through regulating the spatio-temporal expression of downstream genes.The interaction between transcription factors and DNA is a key section in the process of exploring transcriptional regulatory networks.In the past few years,researchers utilize yeast one hybrid(Y1H)and electrophoresis mobility shift assay(EMSA)to examine whether a transcription factor directly interacts with target DNA.In addition,transient luciferase activity assay provides a convenient method for researchers to test the regulation of transcription factors on downstream gene expression.In this paper,we elaborate the principles,methods,and advantages and limitations of Y1 H,EMSA and transient luciferase activity assay,to provide technical references for exploring the transcription factor-DNA interactions.

关 键 词：转录因子 DNA Y1H EMSA 瞬时表达技术 

分 类 号：Q943.2[生物学—植物学]