血三七正丁醇部位化学成分的研究  

Chemical constituents from the n-butanol fraction of Polygonum amplexicaule

作　　者：赵凡 戴湾 李翎熙 李漓 蔡佳 肖玉琴 许光明[1] 周小江[1] ZHAO Fan;DAI Wan;LI Ling-xi;LI Li;CAI Jia;XIAO Yu-qin;XU Guang-ming;ZHOU Xiao-jiang(Hunan University of Chinese Medicine,Changsha 410208,China;Yueyang Manicipal Hospital of Traditional Chinese Medicine,Yueyang 414000,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]岳阳市中医院,湖南岳阳414000

出　　处：《中成药》2020年第9期2346-2350,共5页Chinese Traditional Patent Medicine

基　　金：湖南省科技计划项目(2015JC3074);湖南省中药饮片标准化及功能工程技术研究中心项目(BG201803);湖南省大学生研究性学习和创新实验计划课题(201824);湖南中医药大学一层次学科中药学资助(2018-3)。

摘　　要：目的研究血三七(Polygonum amplexicaule D.Don var.Sinense Forbes et Hemsl)正丁醇部位的化学成分。方法血三七70%乙醇提取物正丁醇部位采用薄层色谱、正相硅胶、反相硅胶、大孔树脂、Sephadex LH-20、制备薄层、半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到11个化合物,分别鉴定为大黄素(1)、槲皮素(2)、阿魏酸(3)、对羟基苯乙醇(4)、胡萝卜苷(5)、白藜芦醇(6)、苯甲醇-O-β-D-吡喃葡萄糖苷(7)、对甲氧基甲苯(8)、(1R,2R,4S)-p-menthane-1,2,8-triol 1-O-β-D-glucopyranoside(9)、bergenin-11-O-p-hydroxybenzoate(10)、11-O-(4′-O-methylgalloyl)-bergenin(11)。结论化合物6~11均为首次从该植物中分离得到。AIM To study the chemical constituents from n-butanol fraction of Polygonum amplexicaule D.Don var.sinense Forbes et Hemsl.METHODS The n-butanol fraction of 70%ethanol extract extract from P.amplexicaule was isolated and purified by TLC,normal-phase silica,reverse silica,macroporous resin,Sephadex LH-20,preparative TLC and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Eleven compounds were isolated and identified as emodin(1),quercetin(2),ferulic acid(3),p-hydroxyphenyl ethanol(4),daucosterol(5),resveratrol(6),benzyl alcohol-O-beta-D-glucopyranoside(7),p-methoxytoluene(8),(1R,2R,4S)-p-menthane-1,2,8-triol 1-O-β-D-glucopyranoside(9),bergenin-11-O-p-hydroxybenzoate(10),11-O-(4′-O-methylgalloyl)-bergenin(11).CONCLUTION Compounds 6-11 are isolated from this plant for the first time.

关 键 词：血三七 正丁醇部位 化学成分 分离鉴定 

分 类 号：R284.1[医药卫生—中药学]