Wenyang Huazhuo Fang exerts transient receptor potential cation channel subfamily C member-dependent nephroprotection in a rat model of doxorubicin-induced nephropathy  

作　　者：An Peng Dong Sheng Li Xingyao Cai Zimo Ye Bingyu Zhang Aijun Shi Xingmin Wu Xili 

机构地区：[1]Department of Integrated Chinese Traditional and Western Medicine,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China [2]Department of Nephropathy,the Affiliated Hospital of Shaan Xi University of Chinese Medicine,Xianyang 712023,China [3]Department of Pharmacy,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710082,China [4]Department of Immunology,Xi'an Jiaotong University Health Science Center,Xi'an 710061,China

出　　处：《Journal of Traditional Chinese Medicine》2020年第4期613-620,共8页中医杂志（英文版）

基　　金：the Key Research and Development Projects of Shaanxi Province(Study on the Effect of Wenyanghuazhuo Method on Autophagy-related Proteins in Adriamycin Nephropathy Rat Model,No.2020SF-340);a Grant from the Sci-tech Project of Shaanxi Province(Study on the Expression Regulation of STIM1/Orail and TRPC-related Channel Proteins in Renal Tissues of Adriamycin Nephropathy Rats by Aconiti Lateralis Radix and Wenyanghuazhuofang,No.2016SF-068)。

摘　　要：OBJECTIVE:To determine the effect of Wenyang Huazhuo Fang(WHF),a Traditional Chinese Medicine decoction,on renal function in a rat model of doxorubicin-induced nephropathy,and to elucidate the underlying mechanism.METHODS:Sprague-Dawley rats were randomly divided into six groups:control,doxorubicin-nephropathy,and prednisone-treated(6.45 mg·kg^-1·d^-1)doxorubicin nephropathy groups,as well as high-(7.26 g·kg^-1·d^-1),medium-(2.42 g·kg-1·d-),and low-dose(0.81 g·kg^-1·d^-1)WHF-treated doxorubicin-nephropathy groups.The nephropathy rat model was established by two tail vein injections of doxorubicin,followed by prednisone or WHF treatment for 8 weeks.Body weights were monitored and urinary protein was measured every 2 weeks.After the end of the treatment period,the rats were euthanized.Serum biochemical indicators were determined and renal morphological alterations were assessed using histological staining.The expression of transient receptor potential cation channel subfamily C member 6(TRPC6),stromal interaction molecule 1(STIM1),and calcium release-activated calcium channel protein 1(Orai1)was detected using western blotting,and their mRNA levels were examined using quantitative real-time reverse transcription-polymerase chain reaction.RESULTS:WHF treatment was found to significantly ameliorate weight loss,proteinuria,hypoalbuminemia,and dyslipidemia in doxorubicin-nephropathy rats.The protein and mRNA levels of TRPC6,STIM1,and Orai1 were partially,but significantly suppressed by prednisone or WHF treatment.CONCLUSION:Treatment with WHF significantly ameliorates renal injury in a rat model of doxorubicin-induced nephropathy,which could be at least partially related to repression of the TRPC6 pathway.

关 键 词：NEPHROPATHY Doxorubicin TRPC6 cation channel Stim1 protein rat Orai1 protein rat Wenyang Huazhuo Fang 

分 类 号：R285.5[医药卫生—中药学]