解决ELISA技术应用过程中OD值精确度偏低的研究  

Research on Solving the Low Accuracy of OD Value in the Application of ELISA Technology

作　　者：杨大明 张发胜 Yang Daming;Zhang Fasheng(Hongyuan Qimu Co.Ltd.of Jiuquan Iron and Steel(Group)Corporation,Jiayuguan,Gansu,735100)

机构地区：[1]酒钢集团宏源公司祁牧乳业有限责任公司,甘肃嘉峪关735100

出　　处：《酒钢科技》2020年第3期30-34,共5页

摘　　要：通过对ELISA技术应用过程中试剂回温、加样方式、反应温度、洗液回温、缩短加样时间等措施的调整,统计分析调整前后OD(光密度)值的变化趋势及平行组间的相对误差,确定检测结果的准确度,为ELISA技术在早孕、牛副结核、病毒性腹泻、传染性胰脏坏死病毒、甲肝病毒等方面的应用提供技术支持,同时可以较少检测误差带来的经济风险。By adjusting the temperature of reagent,the way of adding sample,the temperature of reaction,the temperature of washing solution and shortening the time of adding sample,the change trend of OD (optical density) value before and after adjustment and the relative error between parallel groups were statistically analyzed to determine the accuracy of the detection results,which provide technical support for the application of ELISA technology in early pregnancy,paratuberculosis,viral diarrhea,infectious pancreatic necrosis virus and hepatitis a virus etc..At the same time,it can reduce the economic risk caused by detection error.

关 键 词：ELISA技术 OD值 相对误差 

分 类 号：R51[医药卫生—内科学]