基于BSA-seq法的油菜野芥胞质雄性不育恢复基因的分析  

Location Analysis of Restorer Gene of Sinapis Arvensis Cytoplasmic Male Sterility in Brassica napus Based on BSA-Seq Method

作　　者：尹明智[1] 胡燕[1] YIN Mingzhi;HU Yan(College of Biology and Agricultural Science&Technology, Zunyi Normal University, Zunyi, Guizhou 563006, China)

机构地区：[1]遵义师范学院生物与农业科技学院,贵州遵义563006

出　　处：《西北植物学报》2020年第7期1148-1156,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31501337);贵州省科学技术厅、遵义市科学技术局、遵义师范学院联合科技基金(黔科合LH字[2016]7014号);贵州省高层次创新型人才培养计划(遵市科合人才[2017]11号)。

摘　　要：细胞质雄性不育是利用杂种优势创制油菜杂交种的重要途径之一。而恢复系的选育及恢复基因的研究有利于提高细胞质雄性不育系的应用价值。该研究以甘蓝型油菜细胞质雄性不育系1193A和恢复系15-R1为亲本,以F2群体构建了2个极端性状混池,采用BSA-seq技术方法,对恢复基因进行定位分析。结果表明:(1)不育系1193A与恢复系15-R1之间共获得非同义突变的SNPs共33883个,混池间共有7996个非同义编码突变。(2)InDel检测与注释结果表明,亲本间引起移码突变的有2918个,混池间引起移码突变的有840个。(3)综合SNP和InDel的关联分析结果,将恢复基因定位于C09染色体上的0~880000区域,总长度为880 kb。(4)对候选区域的SNP和InDel注释发现,亲本间存在非同义突变的SNP共40个,混池间存在非同义突变的SNP共65个,亲本间存在移码突变的InDel共7个,混池间存在移码突变的InDel共11个;对候选区间内的编码基因进行注释,结果共注释到162个基因,其中存在非同义突变基因11个,移码突变基因5个,这些基因可能与育性恢复有关。Cytoplasmic male sterility(CMS)was one of the important ways to create hybrid by using heterosis in Brassica napus.The selection of restorer line and the study of restorer gene was helpful to improve the application value of CMS line.In this study,we used the cytoplasmic male sterile line 1193A and restorer line 15-R1 of Brassica napus as parents to construct a hybrid pool of two extreme characters in F2 population.The restorer gene was located and analyzed by BSA-seq technology.The results showed that:(1)33883 non-synonymous SNPs between 1193A and 15-R1,and 7996 non-synonymous coding mutations between mixed pools were obtained.(2)The results of indel detection and annotation showed that there were 2918 mutations between parents and 840 mutations between mixed pools.(3)Based on the correlation analysis of SNP and indel,the restorer gene was located in the 0-880000 region of C09 chromosome,with a total length of 880 kb.(4)Through the SNP and indel annotation of candidate regions,we found that there were 40 SNPs with non-synonymous mutations between parents,65 SNPs with non-synonymous mutations between mixed pools,7 indels with frame shifting mutations between parents and 11 indels with frame shifting mutations between mixed pools.The coding genes in the candidate region were annotated.The results showed that 162 genes were annotated,including 11 non-synonymous mutations and 5 frame shifting mutations.These genes may be related to fertility restoration.

关 键 词：油菜 野芥细胞质雄性不育 恢复基因 基因组测序 BSA法 

分 类 号：Q789[生物学—分子生物学]