可溶性HLA-G和Toll样受体2、Rta基因在传染性单核细胞增多症中的表达及相关性分析  

Expression and correlation analysis of soluble HLA-G,Toll like receptor 2 and Rta gene in infectious mononucleosis

作　　者：韩红满 秦伟[1] 左立辉[1] 刘贵敏 李四强[1] Han Hongman;Qin Wei;Zuo Lihui;Liu Guimin;Li Siqiang(Department of Endocrinology, Hospital of the 82nd Army Group of the Chinese People's Liberation Army, Baoding 071000, China)

机构地区：[1]中国人民解放军陆军第八十二集团军医院血液内分泌科,河北省保定071000

出　　处：《疑难病杂志》2020年第9期913-916,共4页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省保定市科学技术研究与发展指导计划项目(17ZF273)。

摘　　要：目的分析可溶性人类白细胞抗原-G(sHLA-G)和Toll样受体2(TLR2)、EB病毒复制转录激活子(Rta)基因在传染性单核细胞增多症中的表达及相关性。方法选取2018年1月—2019年12月中国人民解放军陆军第八十二集团军医院血液内分泌科诊治传染性单核细胞增多症患儿80例作为观察组,另外选取同期于医院行健康体检的80例健康儿童作为健康对照组,采集健康对照组、观察组(急性期、恢复期)血液样本,检测sHLA-G、TLR2、Rta表达,并分析三者相关性。结果观察组sHLA-G表达水平及TLR2、Rta mRNA表达量均高于健康对照组(t/P=47.670/0.001、44.640/0.001、22.700/0.001)。观察组sHLA-G、TLR2 mRNA、Rta mRNA表达量末期>后期>中期>前期(F/P=7.866/0.001、42.680/0.001、33.681/0.001),sHLA-G、TLR2 mRNA、Rta mRNA表达量急性期均高于恢复期(t/P=10.390/0.001、19.880/0.001、27.000/0.001)。sHLA-G与TLR2、Rta mRNA之间均呈正相关(r/P=0.404/0.001、0.240/0.032),TLR2 mRNA与Rta mRNA呈正相关(0.271/0.015)。结论sHLA-G、TLR2 mRNA、Rta mRNA在传染性单核细胞增多症中均为异常高表达,且三者相关,共同参与该病的发生发展。Objective To analyze the expression and correlation of soluble human leukocyte antigen G(sHLA-G),Toll like receptor 2(TLR2)and Epstein Barr virus replication and transcription activator(Rta)gene in infectious mononucleosis.Methods From January 2018 to December 2019,80 cases of children with infectious mononucleosis diagnosed and treated in the Department of Hematology and Endocrinology of the 82nd Group Military Hospital of the People's Liberation Army were selected as the observation group,and 80 healthy children who underwent physical examination in the hospital during the same period were selected as the healthy control group.Blood samples of the healthy control group and the observation group(acute phase and recovery period)were collected to detect sHLA-G.The expression of TLR2 and Rta was analyzed.Results The expression levels of sHLA-G,TLR2 and Rta mRNA in the observation group were higher than those in the healthy control group(t/P=47.670/0.001,44.640/0.001,22.700/0.001).In the observation group,the expression of sHLA-G,TLR2 mRNA and Rta mRNA in the late stage>the late stage>the early stage(F/P=7.866/0.001,42.680/0.001,33.681/0.001).The expression levels of sHLA-G,TLR2 mRNA and Rta mRNA in the acute phase were higher than those in the recovery stage(t/P=10.390/0.001,19.880/0.001,27.000/0.001).SHLA g was positively correlated with TLR2 and Rta(r/P=0.404/0.001,0.240/0.032),and TLR2 was positively correlated with Rta(0.271/0.015).Conclusion sHLA-G,TLR2 and Rta are all abnormally high expression in infectious mononucleosis,and they are related to each other and participate in the occurrence and development of infectious mononucleosis.

关 键 词：可溶性人类白细胞抗原-G TOLL样受体2 EB病毒复制转录激活子 传染性单核细胞增多症 相关性 

分 类 号：R71[医药卫生—妇产科学]