祛瘀生肌方含药血清对人真皮微血管内皮细胞迁移和PGT相关因子的影响  

Effects of Quyu Shengji Formula Containing Serum on the Migration of Human Dermal Microvascular Endothelial Cells and Expressions of PGT-related Factors

作　　者：卢怡 丁晓杰 祁菲 陈曦[1] 蒯仂 周蜜 李苏[1] 杨滢瑶 茹意 李福伦[1] 迮侃[1] LU Yi;DING Xiao-jie;QI Fei;CHEN Xi;KUAI Le;ZHOU Mi;LI Su;YANG Ying-yao;RU Yi;LI Fu-lun;ZE Kan(Department of Dermatology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai Hospital of Traditional Chinese Medicine,Institute of Dermatology,Shanghai Academy of Traditional Chinese Medicine,Shanghai,200437;Department of Vascular,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai,200082)

机构地区：[1]上海中医药大学附属岳阳中西医结医院皮肤科上海市中医药研究院皮肤病研究所,上海200437 [2]上海中医药大学附属中西医结合医院脉管科,上海200082

出　　处：《中国中西医结合杂志》2020年第9期1088-1093,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金青年项目(No.81704085,No.81904214,No.81673886);国家重点研发计划中医药现代化研究重点专项(No.2018YFC1705301);上海市进一步加快中医药事业发展三年行动计划(No.ZY(2018-2020)-FWTX-4010);2019上海中医药大学研究生“研究生创新培养”专项科研项目(No.Y2019027)。

摘　　要：目的探讨祛瘀生肌方对人真皮微血管内皮细胞(HDMECs)迁移能力影响和前列腺素转运蛋白(PGT)相关因子的作用机制。方法将HDMECs细胞分为5组。低糖空白组(A组):细胞中加5mmol/L葡萄糖+10%空白血清;高糖空白组(B组):细胞中加25mmol/L葡萄糖+10%空白血清;生肌药组(C组):细胞中加25mmol/L葡萄糖+10%生肌药含药血清;化瘀药组(D组):细胞中加25mmol/L葡萄糖+10%化瘀药含药血清;生肌化瘀药组(E组):细胞中加25mmol/L葡萄糖+10%生肌化瘀药含药血清。采用Transwell、划痕实验、ELISA、Western Blot检测含药血清干预后HDMECs的迁移能力和PGT相关因子的表达。结果Transwell结果示:与B组比较,C、D、E组的细胞迁移能力明显升高(P<0.05)。划痕实验0、12、24 h显示,D组未愈合面积进一步缩小(P<0.05)。ELISA结果示C、D、E组的前列腺素E2(PGE2)含量均有所增加,其中D组的PGE2含量最接近低A组。Western Blot结果示:与B组比较,给药后C、D、E组PGT含量均显著降低(P<0.05),细胞内皮生长因子(VEGF)分泌量均显著增加(P<0.05),D组增加量最为显著(P<0.01)。结论祛瘀生肌方含药血清可提高HDMECs迁移能力,促进HDMECs分泌PGE2、VEGF,并降低PGT含量。Objective To observe the effects and the mechanism of Quyu Shengji Formula(QYSJF)on the migration of human dermal microvascular endothelial cells(HDMECs)and expressions of prostaglandin transporter(PGT)-related factors.Methods HDMECs were divided into 5 groups:low glucose blank group(A)[b y adding 5 mmol/L glucose+10%vehicle serum],high glucose blank group(B)[b y adding 25 mmol/L glucose+10%vehicle serum],Shengji Formula group(C)[by adding 25 mmol/L glucose+10%Shengji Formula containing serum],Quyu Formula group(D)[by adding 25 mmol/L glucose+10%Quyu Formula containing se ru m],QYSJF group[b y adding 25 mmol/L glucose+10%QYSJF containing se ru m].Effects of serum containing QYSJF on the migration ability of HDMECs and the expressions of PGT-related factors were detected by Transwell experiment,scratch test,ELISA,and Western Blot.Results Transwell results showed that compared with Group B,the cell migration ability significantly increased in Group C,D,and E(P<0.05).The scratch test at 0,12,and 24 h showed that the unhealed area in Group D was further reduced(P<0.05).ELISA results showed that the content of PGE2 increased in Group C,D,and E.Of them,PGE2 content in Group D was the closest to Group A.Western Blot results showed that the PGT content was significantly lower after administration in Group C,D,and E,as compared with Group B(P<0.05).Compared with Group B,the secretion of vascular endothelial growth factor(VEGF)increased significantly after administration in Group C,D,and E(P<0.05),and the increase was the most significant in Group D(P<0.01).Conclusion QYSJF containing serum improved the migration ability of HDMECs,promoted its secretions of PGE2 and VEGF,and decreased the content of PGT.

关 键 词：祛瘀生肌方 糖尿病性溃疡 人真皮微血管内皮细胞 前列腺素转运蛋白 前列腺素E2 血管内皮生长因子 迁移能力 

分 类 号：R285[医药卫生—中药学]