HPLC法测定蒙药红花清肝十三味丸(HHQG)的胆红素含量  

Determination of bilirubin in Mongolian medicine Honghua qinggan Shisanwei Pill(HHQG)by HPLC

作　　者：董珉翔 虎钦 杨宏昕 郭小彬 郭浩 王艳丽 常亮 陈广荣 DONG Min-xiang;HU Qin;YANG Hong-xin;GUO Xiao-bin;GUO Hao;WANG Yan-li;CHANG Liang;CHEN Guang-rong(The first hospital of Inner Mongolia autonomous region bureau of prisons,Huhehot010050,China;Inner Mongolia Kaimeng Wuliaotang research institute of traditional Mongolian and Chinese medicine,Huhehot 10070 China;The Inner Mongolia Autonomous Region People′s Hospital,Huhehot 010017,China;The Inner Mongolia Autonomous Region Hospital of International Mongolian Medicine,Huhehot 010065,China;People's Hospital Affiliated to Medical University of Inner Mongolia Autonomous Region,Huhehot 010010,China)

机构地区：[1]内蒙古自治区监狱管理局第一医院,内蒙古呼和浩特010050 [2]内蒙古凯蒙五疗堂蒙中医药研究院,内蒙古呼和浩特010070 [3]内蒙古自治区人民医院,内蒙古呼和浩特010017 [4]内蒙古国际蒙医医院,内蒙古呼和浩特010110 [5]内蒙古医科大学附属人民医院,内蒙古呼和浩特010010

出　　处：《中国民族医药杂志》2020年第8期46-48,共3页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：内蒙古自治区自然科学基金(编号:2016MS0871);国家自然科学基金项目(编号:81260659)。

摘　　要：目的:HPLC法测定蒙药红花清肝十三味丸(HHQG)的胆红素含量。方法:色谱柱C18(4.6 mm×200 mm,5μm);流动相甲醇-乙腈-1%冰乙酸(88∶10∶2);流速1 mL·min^-1;测定波长453 nm。结果:胆红素0.0118~0.472μg成线性关系(r=0.999);胆红素平均质量分数0.104 mg·g^-1。结论:本法测定胆红素的含量,操作简便,准确可靠,可用于药品的质量控制。Objective:To determine the content in HHQG bilirubin by HPLC.Methods:The chromatographic column was C18(4.6 mm×200 mm,5 μm),mobile phase methanol-acetonitrile-1% glacial acetic acid(88∶10∶2),the velocity of 1 mL·min^-1,the measuring wavelength 453 nm.Results:The bilirubin linear relationship was between 0.0118 ~0.472 μg(r =0.999).The average mass fraction of bilirubin was 0.104 mg·g^-1.Conclusion:The method is simple, accurate and reliable for the determination of bilirubin in HHQG.

关 键 词：红花清肝十三味丸 HHQG 胆红素 HPLC方法 

分 类 号：R291.2[医药卫生—民族医学]