黔产金钗石斛茎和花中浸出物测定  

Determination of extracts from stems and flowers of Dendrobium nobile from Guizhou

作　　者：杨婷婷 钟可 刘秀平 黄芯琦 韩楷 YANG Tingting;ZHONG Ke;LIU Xiuping;HUANG Xinqi;HAN Kai(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025

出　　处：《贵州科学》2020年第5期39-43,共5页Guizhou Science

摘　　要：目的:测定黔产金钗石斛茎和花中浸出物的含量。方法:按照2015版《中国药典》四部(通则2201)浸出物测定法项下,对水溶性和醇溶性浸出物测定法中的冷浸法和热浸法进行实验,优选金钗石斛的最佳浸出物测定法,并对金钗石斛茎和花的浸出物进行测定。结果:金钗石斛的浸出物测定以醇溶性热浸法为佳,溶剂为20%乙醇。不同采收月份的金钗石斛茎中浸出物的含量以2月份平均含量最高,为35.14%,8月份平均含量最低,为16.29%;不同生长年限的金钗石斛茎中浸出物的含量:一年生>三年生>二年生,其中一年生者浸出物含量为27.33%。不同干燥方式的金钗石斛花中浸出物的含量:阴干>60℃烘干>30℃烘干>晒干。结论:黔产金钗石斛浸出物测定实验结果表明,2月份采收一年生金钗石斛茎质量最佳,金钗石斛花的干燥方法以阴干最好。In this study we determined the extracts from the stems and flowers of Dendrobium nobile from Guizhou.According to the extract determination method in the Chinese Pharmacopoeia,the cold-/hot-leaching method for the determination of water-/ethanol-soluble extracts was tested to optimize the method for the determination of extracts from Dendrobium nobile. The ethanol-soluble-hot-leaching method was proved to be the best method for the determination of extracts from Dendrobium nobile , and the solvent was 20% ethanol. The content of extracts from the stems of Dendrobium nobile varied among different harvesting months, and it was the highest in February (35.14%) and the lowest in August (16.29%). The content of extracts from the stems of Dendrobium nobile of different growing years ranked as follows: annuals>triennials>biennials;the content of extracts in the annuals was 27.33%. The content of extracts from the flowers of Dendrobium nobile by different drying methods ranked as follows: drying in the shade>60 ℃ drying>30 ℃ drying>drying in the sun. The results showed that the quality of the stems of annual Dendrobium nobile harvested in February was the best, and drying in the shade was the best drying method for Dendrobium nobile flowers.

关 键 词：金钗石斛茎 金钗石斛花 浸出物 不同月份 不同年限 

分 类 号：R284.1[医药卫生—中药学]