花生茎线虫的脱水存活方式及耐低温能力  

Anhydrobiosis and low temperature tolerance of Ditylenchus arachis

作　　者：陈思怡 邓明雪 陈晶伟 方伟琦 刘国坤[1] 肖顺[1] 程曦 CHEN Siyi;DENG Mingxue;CHEN Jingwei;FANG Weiqi;LIU Guokun;XIAO Shun;CHENG Xi(Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2020年第5期577-582,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金——青年科学基金项目(31501615、31201495);福建农林大学科技创新专项基金项目(CXZX2019012G).

摘　　要：通过室内试验,对花生茎线虫经快速脱水干燥、缓慢脱水干燥及低温处理后的存活情况进行研究.结果表明:花生茎线虫的脱水存活方式为缓慢脱水策略型;经缓慢脱水处理后,其不同虫态的耐干燥能力存在差异:3龄+4龄幼虫>2龄幼虫>成虫.将花生茎线虫直接置于低温(-10和-20℃)下保存7 d后死亡率达85%以上,但经缓慢脱水处理后的线虫在-10、-20和-80℃下保存35 d后,复苏率分别为18.7%、16.0%和31.8%.To reveal the anhydrobiosis strategy and low temperature tolerance of Ditylenchus arachis,survival rates of D.arachis were investigated under fast and slow dehydration,and low temperature stress.The results showed that the survival strategy of D.arachis belonged to slow dehydration.Its tolerance to dehydration varied significantly among different stages of nematode,whose order was 3rd-and 4th-instar larvae>2nd-instar larvae>adult.Further study showed that the death rate of D.arachis exceeded 85%after cryopreservation at-10 and-20℃for 7 days,though the survival rate of nematodes was 18.7%,16.0%and 31.8%,respectively after slow dehydration at-10,-20 and-80℃for 35 d.

关 键 词：花生茎线虫 花生 脱水策略 抗逆性 

分 类 号：S432.4[农业科学—植物病理学]