甘薯薯瘟病菌RPA检测方法的建立及应用  

Establishment and application of RPA detection for bacterial wilt of sweet potato caused by Ralstonia solanacearum

作　　者：李华伟[1] 林志坚[1] 张鸿[1] 刘中华[1] 李国良[1] 汤浩[1] 邱思鑫[1] LI Huawei;LIN Zhijian;ZHANG Hong;LIU Zhonghua;LI Guoliang;TANG Hao;QIU Sixin(Crop Research Institute,Fujian Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture/Technical Research Center of Specialty Dry Crop Variety Breeding of Fujian,Fuzhou,Fujian 350013,China)

机构地区：[1]福建省农业科学院作物研究所/农业部南方薯类科学观测实验站/福建省特色旱作物品种选育工程技术研究中心,福建福州350013

出　　处：《福建农林大学学报（自然科学版）》2020年第5期583-588,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省公益类科研专项(2017R1026-4);现代农业产业技术体系(CARS-10-B14);福建省农业科学院创新团队项目(STIT2017-2-3).

摘　　要：根据甘薯薯瘟病菌特异基因(orf428)序列设计重组酶聚合酶等温扩增(RPA)引物,通过特异性和灵敏性验证,建立了能够快速检测甘薯薯瘟病菌的RPA方法.该方法检测结果与PCR检测结果一致,且灵敏度高、特异性强,在39℃的恒温条件下反应20 min即可完成核酸扩增,大大提高了检测效率.此外,该方法可用于检测甘薯发病组织、带菌土壤和水体中的薯瘟病菌.In order to develop a quick detection method for sweet potato bacterial wilt arising from Ralstonia solanacearum,specific primers for recombinase polymerase amplification(RPA)were designed from the specific gene(orf428)of sweet potato bacterial wilt pathogen.The results of RPA were consistent with those of PCR,with high sensitivity and specificity.It only took 20 minutes to complete nucleic acid amplification at 39℃,which greatly improved the detection efficiency.Moreover,the RPA method can be applied to detect infected sweet potato tissue,soil and water body.

关 键 词：甘薯 薯瘟病 青枯菌 重组酶聚合酶等温扩增方法 

分 类 号：S531[农业科学—作物学] S411