云南小麦品种(系)抗逆性相关基因的KASP标记检测  

Identification of Genes Associated with Stress Resistance in Yunnan Wheat Cultivars(Lines)by KASP Assays

作　　者：王志伟[1] 乔祥梅[1] 王志龙[1] 杨金华[1] 程加省[1] 程耿[1] 于亚雄[1] WANG Zhi-wei;QIAO Xiang-mei;WANG Zhi-long;YANG Jin-hua;CHENG Jia-sheng;CHENG Geng;YU Ya-xiong(Food Crops Research Institute,Yunnan Academy of Agricultural Sciences/Yunnan Branch,National Wheat Improvement Center,Yunnan Kunming 650200,China)

机构地区：[1]云南省农业科学院粮食作物研究所/国家小麦改良中心云南分中心,云南昆明650200

出　　处：《西南农业学报》2020年第8期1601-1607,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划“七大农作物育种”重点专项(2017YFD0100904);国家小麦现代产业技术体系昆明综合试验站(CARS-3-2-43);云南省现代农业麦类产业技术体系。

摘　　要：【目的】明确云南小麦品种中抗旱和抗穗发芽等抗逆性状相关基因的组成分布,为云南小麦抗逆育种提供理论依据。【方法】利用3个抗旱和3个抗穗发芽相关基因的KASP标记,对云南育成的42份小麦品种(系)进行基因型检测。【结果】23份品种(系)为TaDreb-B1基因的TaDREB-B1a耐旱单倍型,频率为54.76%;24份品种(系)为1-feh w3基因的Westonia type抗旱单倍型,频率为57.14%;35份品种(系)为TaCwi-4A基因的Hap-4A-C抗旱单倍型,频率为83.33%。29份品种(系)为TaVp-1B基因的Vp1B1c抗穗发芽单倍型,频率为69.05%;35份品种(系)为TaPHS_646基因的RioBlanceo type抗穗发芽单倍型,频率为83.33%;17份品种(系)为TaSdr-B1基因的TaSdr-B1a抗穗发芽单倍型,频率为40.48%。11份品种(系)为3个抗旱基因的抗性单倍型组合,频率为26.19%;10份品种(系)为3个穗发芽抗性基因的抗性单倍型组合,频率为23.81%;2份品种(云麦76、保麦2号)为3个抗旱性和3个穗发芽抗性相关基因的有利单倍型组合,频率为4.76%。【结论】充分利用这些含有优异抗逆基因资源的品种(系),在云南小麦抗逆育种中聚合应用上述基因有利单倍型的,可有效的综合提高云南小麦抗逆性。【Objective】In order to provide a theoretical basis for wheat stress resistance breeding in Yunnan Province,the present study was used to identify genotypes of functional genes related to drought tolerance and pre-harvest sprouting(PHS)resistance in Yunnan wheat cultivars.【Method】With KASP assays of 3 genes related to drought tolerance and 3 genes related to PHS resistance,42 Yunnan wheat cultivars(lines)were genotyped.【Result】The results showed that on drought tolerance,23 cultivars(lines)with a frequency of 54.76%were TaDREB-B1a favorable haplotype of TaDreb-B1 gene,24 cultivars(lines)with a frequency of 57.14%were Westonia type favorable haplotype of 1-feh w3 gene,35 cultivars(lines)with a frequency of 83.33%were Hap-4A-C favorable haplotype of TaCwi-4A gene.On PHS resistance,29 cultivars(lines)with a frequency of 69.05%were Vp1B1c favorable haplotype of TaVp-1B gene,35 cultivars(lines)with a frequency of 83.33%were RioBlanceo type favorable haplotype of TaPHS1 gene,17 cultivars(lines)with a frequency of 40.48%were TaSdr-B1a favorable haplotype of TaSdr-B1 gene.11 cultivars(lines)with a frequency of 26.19%carried favorable haplotype combinations of 3 drought tolerant genes,10 cultivars(lines)with a frequency of 23.81%carried favorable haplotype combinations of 3 PHS resistance genes.Two cultivars(Yumai76 and Baomai2)carried favorable haplotype combinations of 3 drought tolerant genes and 3 PHS resistance genes,with a frequency of 4.76%.【Conclusion】Pyramiding a number of favorable haplotypes of functional gene as TaDREB-B1a,Westonia type,Hap-4A-C,RioBlanceo type,TaSdr-B1a,and Vp1B1c was the effective way to comprehensively improve the stress resistance of Yunnan wheat cultivars.

关 键 词：小麦 基因 抗旱性 穗发芽抗性 KASP检测 

分 类 号：S512[农业科学—作物学]