ZnO电致化学发光以及对尿酸的检测研究  

An electrochemiluminescent sensor for the determination of uric acid based on ZnO

作　　者：郑瑞娟[1] 江道鸿 陈江海 林思伟 陈怀义 ZHENG Rui-juan;JIANG Dao-hong;CHEN Jiang-hai;LIN Si-wei;CHEN Huai-yi(College of Chemistry and Materials Science,Longyan University,Longyan 364012,China)

机构地区：[1]龙岩学院化学与材料学院,福建龙岩364012

出　　处：《化学研究与应用》2020年第9期1601-1607,共7页Chemical Research and Application

基　　金：福建省教育厅中青年项目[JAT170564]资助。

摘　　要：通过直接沉淀法制备纳米ZnO,采用SEM和XRD技术对纳米ZnO进行了表征。纳米ZnO在以K2S2O8作为共反应剂的NaOH溶液中具有良好的电致化学发光现象。对电致化学发光的影响因素进行了研究,并对发光机理进行了讨论。基于尿酸对ZnO的电化学发光强度有猝灭作用,建立了一种测定尿酸含量的方法。在优化实验条件下,尿酸浓度的对数在2×10^-9~8×10^-7 mol·L^-1范围内与电致化学发光强度具有良好的线性关系,检测限为1×10^-9 mol·L^-1。该方法具有操作简便,检测时间短,稳定性好等优点,并成功应用于人体尿液中尿酸含量的的检测分析。In the present work,ZnO nanoparticles(ZnONPs)were successfully synthesized by direct precipitation method,and characterized by scanning electron microscopy and X-ray diffraction.The ZnONPs possessed good electrochemiluminescence(ECL)behavior in NaOH aqueous in the presence of K 2S 2O 8 as coreaction reagent.The influence factors such as scanning speed,concentrations of K 2S 2O 8 and concentrations of NaOH were investigated,and luminescence mechanism of the ECL behavior of ZnONPs was further studied.A novel ECL sensor method for determination of uric acid was established based on the quenching effect of the electrochemical luminescence of ZnONPS.Under the optimal conditions,the electrochemiluminescence intensity showed a good linear dependence with the logarithm of the concentration of uric acid over the range between 2×10^-9 mol·L^-1 and 8×10^-7 mol·L^-1(R=0.9983)with detection limit of 1×10^-9 mol·L^-1.This method exhibited the advantages of simple operation,short detection time and good stability,and was successfully applied to the determination of uric acid content in human urine.

关 键 词：ZNO 电致化学发光 检测 尿酸 

分 类 号：O657.3[理学—分析化学]