DNA银纳米簇荧光猝灭法测定CTMAB  

Fluorescence quenching detection of CTMAB based on DNA silver nanoclusters

作　　者：刘璨 朱鑫鑫 杨胜园[1,2] 林茜 陈微 肖福兵 梁好 LIU Can;ZHU Xing-xing;YANG Sheng-yuan;LIN Xi;CHEN Wei;XIAO Fu-bing;LIANG Hao(College of Public Health,University of South China,Hengyang 421001,China;Hengyang Key Laboratory of Research on New Technologies for Health Hazard Factor Inspection and Quarantine,Hengyang 421001,China)

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001 [2]衡阳市健康危害因子检验检疫新技术研究重点实验室,湖南衡阳421001

出　　处：《化学研究与应用》2020年第9期1716-1719,共4页Chemical Research and Application

基　　金：国家自然科学基金项目(11205085)资助;湖南省自然科学基金面上项目(2018JJ2323)资助;湖南省衡阳市重点实验室项目(2018KJ110)资助。

摘　　要：本文以发夹型DNA为模板,NaBH 4为还原剂合成了银纳米簇(DNA-AgNCs),并将其作为荧光探针,建立了基于银纳米簇荧光猝灭的快速、灵敏检测阳离子表面活性剂十六烷基三甲基溴化铵(CTMAB)的新方法。利用透射电子显微镜(TEM)对制备的银纳米簇进行表征,结果表明,银纳米簇成球形,具有良好的分散性。进一步研究了DNA-AgNCs的荧光性能,其最大激发波长(Ex)为420 nm、最大发射波长(Em)为525 nm。向银纳米簇溶液加入CTMAB后,DNA-AgNCs的荧光强度明显降低,体系在525 nm处的荧光猝灭程度与CTMAB的浓度在4.0×10^-9~6.0×10^-8 mol·L^-1范围呈现良好的线性关系,检出限为1.3×10^-10 mol·L^-1。A new type of silver nanoclusters(AgNCs)were prepared by using hairpin DNA as template.The as-prepared DNA-AgNCs were characterized through transmission electron microscope and fluorenscence spectroscopy.The DNA-AgNCs exhibited a spherical shape and had good fluorescence intensity at 525 nm.Based on the quenching effected of CTMAB on the fluorescence of DNA-AgNCs,a novel label-free fluorescence assay had been developed for sensitive detection of cetyltrimethylammonium bromide(CTMAB).Under the selected conditions,the quenched fluorescence intensity showed a good linear relationship with the CTMAB concentrations in the range from 4.0×10^-9 mol·L^-1 to 6.0×10^-8 mol·L^-1,the detection limit was 1.3×10^-10 mol·L^-1.

关 键 词：银纳米簇 CTMAB 荧光探针 

分 类 号：O657.39[理学—分析化学]