瞬时受体电位通道C与阻塞性睡眠呼吸暂停低通气综合征大鼠心脏和肾脏损害的关系  

Correlation between transient receptor potential canonical channel with heart and kidney injure of rat model of obstructive sleep apnea hypopnea syndrome

作　　者：温雯 姚巧玲[2] 陈玉岚[1] 李志强[3] 孙晓靖[4] 李瑜[5] 张俊仕[1] 珠勒皮亚·司马义[1] 徐新娟[1] WEN Wen;YAO Qiaoling;CHEN Yulan;LI Zhiqiang;SUN Xiaojing;LI Yu;ZHANG Junshi;SIMAYI Zhulipiya;XU Xinjuan(Department of Hypertension,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China;Department of Physiology,Preclinical Medicine Collage of Xinjiang Medical University,Urumqi 830054,China;Clinical Medical Research Institute,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China;Department of Critical Medicine,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China;Second Department of General Internal Medicine,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学第一附属医院高血压科,新疆维吾尔自治区乌鲁木齐830011 [2]新疆医科大学基础医学院生理教研室,新疆维吾尔自治区乌鲁木齐830054 [3]新疆医科大学第一附属医院临床研究院,新疆维吾尔自治区乌鲁木齐830011 [4]新疆医科大学第二附属医院重症医学科,新疆维吾尔自治区乌鲁木齐830000 [5]新疆医科大学第一附属医院综合内二科,新疆维吾尔自治区乌鲁木齐830011

出　　处：《浙江大学学报（医学版）》2020年第4期439-446,共8页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(82060058)

摘　　要：目的:探究经典瞬时受体电位通道C(TRPC)相关蛋白在阻塞性睡眠呼吸暂停低通气综合征(OSAHS)大鼠心脏和肾脏损害中的作用。方法:18只SD雄性大鼠随机分为实验组和对照组,每组9只。实验组大鼠在间歇性低氧舱中,每天暴露于间歇性低氧环境8 h(10∶00—18∶00)。此后通过实时荧光定量PCR和蛋白质印迹法分别检测大鼠心脏和肾脏组织中TRPC mRNA和相关蛋白的表达。结果:实验组心脏组织中TRPC3、TRPC4、TRPC5的mRNA表达较对照组升高(均P<0.05),而肾脏组织TRPC1、TRPC3、TRPC4、TRPC5、TRPC6、TRPC7的mRNA表达在两组之间差异无统计学意义(均P>0.05);实验组肾脏组织中TRPC4、TRPC5、TRPC6的mRNA表达低于心脏组织(均P<0.05),对照组肾脏组织TRPC7的mRNA表达高于心脏组织(P<0.05)。实验组心脏组织中的TRPC5蛋白表达较对照组升高(P<0.05),而肾脏组织TRPC5、TRPC6、TRPC7相关蛋白的表达在两组之间差异无统计学意义(均P>0.05)。结论:TRPC5可能参与OSAHS心脏损害的病理生理过程,有望成为治疗OSAHS所致心脏损害的药物新靶点。Objective:To investigate the expression of transient receptor potential canonical channels(TRPCs)in the heart and kidney of rat model of obstructive sleep apnea hypopnea syndrome(OSAHS).Methods:Eighteen male SD rats were randomly assigned to intermittent hypoxia(IH)group(n=9)and control group(n=9).In IH group,rats were placed in a chamber and exposed to intermittent hypoxia for 8h(10AM-6PM)daily.The expression of TRPC-related mRNA and protein in the heart and kidney tissue were detected by qRT-PCR and Western blotting,respectively.Results:The mRNA expressions of TRPC3/TRPC4/TRPC5 in heart tissues of IH group were increased significantly compared with the control group(all P>0.05);while there were no significant differences in the mRNA expressions of TRPC1/TRPC3/TRPC4/TRPC5/TRPC6/TRPC7 in kidney tissue between two groups(all P<0.05).The mRNA expressions of TRPC4,TRPC5 and TRPC6 in kidney tissues of IH group were lower than that in heart tissues(all P<0.05).The mRNA expression of TRPC7 in kidney tissues of control group was significantly higher than that in heart tissues(P<0.05).The expression of TRPC5 protein in heart tissues of IH group was significantly higher than that in the control group(P<0.05);while there was no significant differences in the expression of TRPC5/TRPC6/TRPC7 protein in kidney tissue between two groups(all P>0.05).Conclusion:The IH rat model shows that TRPC5 channel is likely to be involved in the OSAHS induced pathophysiological changes in the myocardium and may become a target to prevent OSAHS related cardiac damage.

关 键 词：瞬时受体电位通道/代谢 蛋白质类 睡眠呼吸暂停综合征/病理生理学 低氧/病理学 心脏/代谢 肾/代谢 疾病模型 动物 病例对照研究 

分 类 号：R442.8[医药卫生—诊断学]