内皮蛋白C受体在重度子痫前期孕妇胎盘组织和血浆中表达及意义  

Expression and significance of endothelial protein C receptor in placenta and plasma of pregnant women with preeclampsia

作　　者：马田君[1] 赵秀真 房振亚 MA Tianjun;ZHAO Xiuzhen;PANG Zhenya(Department of Obstetrics and Gynecology,Heze Municipal Hospital Daobeijie Branch,Heze 250014,China;Department of Obstetrics and Gynecology,the Second People's Hospital of Heze,Heze 274000,China;Department of Obstetrics and Gynecology,Shandong Maternal and Child Health Hospital,Jinan 250014,China)

机构地区：[1]菏泽市立医院道碑街院区妇产科,山东菏泽250014 [2]菏泽市第二人民医院妇产科,山东菏泽274000 [3]山东省妇幼保健院妇产科,济南251400

出　　处：《中华实用诊断与治疗杂志》2020年第9期909-912,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(81070511)。

摘　　要：目的探讨重度子痫前期孕妇血浆及胎盘组织中内皮蛋白C受体(endothelial protein C receptor, EPCR)表达变化及临床意义。方法重度子痫前期孕妇78例,其中40例早发型为早发型组,38例晚发型为晚发型组;同孕周接受产检的健康孕妇40例为对照组。采用ELISA法检测3组血浆游离EPCR水平;取3组孕妇分娩胎盘组织,采用免疫组织化学SP法检测结合型EPCR蛋白阳性表达率,实时荧光定量PCR法检测结合型EPCR mRNA相对表达量。结果早发型组胎盘组织结合型EPCR蛋白阳性表达率(57.50%)、结合型EPCR mRNA相对表达量(0.39±0.10)低于晚发型组(92.11%、0.90±0.08)和对照组(97.50%、0.93±0.07)(P<0.05),晚发型组与对照组比较差异无统计学意义(P>0.05)。早发型组血浆游离EPCR水平[(230.56±10.68)μg/L]高于晚发型组[(125.48±7.62)μg/L]和对照组[(122.25±5.65)μg/L],晚发型组与对照组比较差异无统计学意义(P>0.05)。结论晚发型重度子痫前期患者胎盘组织及血浆EPCR无明显变化,早发型重度子痫前期患者胎盘组织结合型EPCR表达减少、血浆游离型EPCR水平升高,可能与其发病有关。Objective To investigate the expression and clinical significance of endothelial protein C receptor(EPCR) in plasma and placenta of pregnant women with severe preeclampsia. Methods Seventy-eight pregnant women with severe preeclampsia included 40 early-onset patients(early-onset group) and 38 late-onset patients(late-onset group), while another 40 healthy pregnant women in the same gestational week were as controls(control group). The level of plasma free EPCR was detected by ELISA. The positive rate of binding EPCR protein in placenta was detected by SP immunohistochemical method. The relative expression of binding EPCR mRNA was detected by real-time fluorescence quantitative PCR technique. Results The positive rate of binding EPCR protein and the relative expression of binding EPCR mRNA were lower in early-onset group(57.50%, 0.39±0.10) than those in late-onset group(92.11%, 0.90±0.08) and control group(97.50%, 0.93±0.07)(P<0.05), and showed no significant differences between late-onset group and control group(P>0.05). The level of free EPCR was higher in early-onset group((230.56±10.68) μg/L) than that in late-onset group((125.48±7.62) μg/L) and control group((122.25±5.65) μg/L), and showed no significant difference between late-onset group and control group(P>0.05). Conclusion The expressions of EPCR in placenta and plasma show no changes in patients with late-onset severe preeclampsia. The expression of binding EPCR in placenta decreases and the level of free EPCR in plasma increases in patients with early-onset severe preeclampsia, which is probably correlated with severe preeclampsia.

关 键 词：重度子痫前期 内皮蛋白C受体 胎盘组织 血浆 

分 类 号：R714.244[医药卫生—妇产科学]