Identification and expression profiles analysis of odorant-binding proteins in soybean aphid,Aphis glycines(Hemiptera:Aphididae)  

作　　者：Ling Wang Ying-Dong Bi Ming Liu Wei Li Miao Liu Shu-Feng Di Shuai Yang Chao Fan Lei Bai Yong-Cai Lai 

机构地区：[1]Institute of Crop Cultivation and Tillage,Heilongjiang Academy of Agricultural Sciences,Harbin,China [2]Heilongjiang Academy of Agricultural Sciences Postdoctoral Program,Harbin,China [3]Virus-free Seedling Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin,China [4]College of Agriculture,Northeast Agricultural University,Harbin,China

出　　处：《Insect Science》2020年第5期1019-1030,共12页昆虫科学（英文版）

基　　金：We gratefully acknowledge Dr.Tao Zhong(Shenyang Academy of Agricultural Sciences,China)for his con-structive reviews of the manuscript.This、work was supported by the Natural Science Foundation of Hei-longjiang(C2018060);Heilongjiang Postdoctoral Fund(LBH-Z16187);Scientific Research Project of Hei-longjiang Academy of Agricultural Sciences(2017ZC10,2017SJ032 and 2018JJPY004);National Key Research and Development Program(2017YFE0111000),National Natural Science Foundation ofChina(31771823);Ma-jor Project of Research and Development of Applied Tech-nology in Heilongjiang Province(GA18B101).

摘　　要：The soybean aphid,Aphis glycines,is an extreme specialist and an important invasive pest that relies on olfaction for behaviors such as feeding,mating,and foraging.Odorant-binding proteins(OBPs)play a vital role in olfaction by binding to volatile compounds and by regulating insect sensing of the environment.In this work we used rapid amplification of complementary DNA ends technology to identify and characterize 10 genes encoding A.glycines OBPs(AglyOBPs)belonging to 3 subfamilies,including 4 classic OBPs,5 Plus-C OBPs,and one Minus-C OBP.Quantitative real-time polymerase chain reaction demonstrated variable specific expression patterns for the 10 genes based on developmental stage and aphid tssue sampled.Expression levels of 7 AglyOBPs(2,3,4,5,7,9,and 10)were highest in the 4th instar,indicating that the 4th nymphal instar is an important developmental period during which soybean aphids regulate feeding and search for host plants.Tissue-specific expression results demonstrated that AglyOBP2,7,and 9 exhibited significantly higher expression levels in antennae.Meanwhile,ligand-binding analysis of5 OBPs demonstrated binding of AglyOBP2 and AglyOBP3 to a broad spectrum of volatiles released by green leaf plants,with bias toward 6-to 8-carbon chain volatiles and strong binding of AglyOBP7 to trans-B-farnesene.Taken together,our findings build a foundation of knowledge for use in the study of molecular olfaction mechanisms and prov ide insights to guide future soybean aphid research.

关 键 词：Aphis glycines expression profiles fluorescence binding assay odorant-binding proteins 

分 类 号：Q96[生物学—昆虫学]