Comparative efficacy of gypsy moth(Lepidoptera:Erebidae)entomopathogens on transgenic blight-tolerant and wild-type American,Chinese,and hybrid chestnuts(Fagales:Fagaceae)  

作　　者：Aaron J.Brown Andrew E.Newhouse William A.Powell Dylan Parry 

机构地区：[1]Department of Environmental and Forest Biology,College of Environmental Science and Forestry,State University of New York,Syracuse,NY,13210,USA

出　　处：《Insect Science》2020年第5期1067-1078,共12页昆虫科学（英文版）

基　　金：Thanks to Hannah Nadel(USDA-APHIS)for kindly providing gypsy moth larvae,and John Podgwaite(USDA Forest Service)for providing purified LdM-NPV.We gratefully acknowledge Seaira Goetz and Elliot Hunsinger for their outstanding technical assistance in the laboratory.Substantive comments by T.Horton and M.K.Fierke improved earlier versions of the manuscript.This project was supported by Biotechnology Risk Assessment Grant Program competitive grant No.2012-33522-19863 from the USDA National Institute of Food and.Agriculture.

摘　　要：American chestnut(Castanea dentata[Marsh.]Borkh.)was once the dominant hardwood species in Eastern North America before an exotic fungal pathogen,Cryphonec-tria parasitica(Murrill)Barr,functionally eliminated it across its range.One promising approach toward restoring American chestnut to natural forests is development of blight-tolerant trees using genetic transformation.However,transformation and related processes can result in unexpected and unintended phenotypic changes,potentially altering ecologi-cal interactions.To assess unintended tritrophic impacts of transgenic American chestnut on plant herbivore interactions,gypsy moth(Lymantria dispar L.)caterpillars were fed leaf disks excised from two transgenic events,Darling 54 and Darling 58,and four control American chestnut lines.Leaf disks were previously treated with an LDso dose of either the speces-specific Lymantria dispar multiple nucleopolyhedrovirus(LdMNPV)or the generalist pathogen Bacillus thuringiensis subsp.kurstaki(Btk).Mortality was quantified and compared to water blank controls.Tree genotype had a strong efect on the efficacies of both pathogens.Larval mortality from Btk-treated foliage from only one transgenic event,Darling 54,differed from its isogenic progenitor,Ellis l,but was similar to an unre-lated wild-type American chestnut control.LdMNPV efficacy was unaffected by genetic transformation.Results suggest that although genetic modification of trees may affect interactions with other nontarget organisms,this may be due to insertion effects,and varia-tion amnon diMterent genvtypes(wlether uasgeic ul wild-typc)iupaults a giealci change in response than transgene presence.

关 键 词：Bacillus thuringiensis LdMNPV NONTARGET restoration tri-trophic unin-tended effects 

分 类 号：Q96[生物学—昆虫学]