Development of a PCR-based method to monitor arthropod dispersal in agroecosystems:Macrolophus pygmaeus(Hemiptera:Miridae)from banker plants to tomato crops  

作　　者：Nuria Agusti Cristina Castane Irene Fraile Oscar Alomar 

机构地区：[1]IRTA(Institute of Agrifood Research and Technology),Cabrils,Barcelona,Spain

出　　处：《Insect Science》2020年第5期1125-1134,共10页昆虫科学（英文版）

基　　金：Thanks to Marta Ramirez for technical support,Montse Matas(ADV Baix Maresme)for her support in pro-viding and managing the greenhouses used and Dr.Priscila Gomez-Polo for her suggestions.This study has been funded by the Spanish Ministry of Economy and Competitiveness(MINECO)(Projects AGL2008-00546,AGL2011-24349 and AGL2014-53970-C2-2-R).Funding acknowledgment also to the CERCA Pro-gramme/Generalitat de Catalunya.

摘　　要：Development of conservation biological control programs requires the identifi-cation of sources that contribute to predator colonization of crops.Macrolophus pygmaeus(Rambur)(Hemiptera:Miridae)is an efficient polyphagous predator used in biological con-trol programs in vegetable crops in Europe.We have developed a marking method based on spraying with a solution of the brine shrimp Artemia spp.(Anostraca:Artemidac)cysts,followed by a PCR detection of Artemia DNA to monitor M.pygmaeus dispersal from banker plants to tomato crops.Experiments conducted in climatic chambers show that the topical application of this marking solution on M.pygmaeus does not significantly reduce adult longevity and that it is detected up to 6 d after the application.When this Artemia solution was applied on Calendula officinalis L.banker plants harboring M.pygmaeus and maintained outdoors,Artemia DNA was still detected on 62%of the insects after6 d.The conducted field applications in commercial greenhouses have confirmed the usefulness of this method to monitor M.pygmaeus dispersal from banker plants to a newly planted tomato crop.This method can be used to assess arthropod movement,being an interesting molecular approach for further improving future pest management strategies.

关 键 词：ARTEMIA arthropod dispersal Calendula officinalis Macrolophus pygmaeus PCR analysis tomato crop 

分 类 号：Q96[生物学—昆虫学]