氮掺杂碳量子点的制备及其在细胞成像与邻硝基苯酚检测中的应用  

Synthesis of Nitrogen-doped Carbon Quantum Dots and Their Applications in Cellular Imaging and o-Nitrophenol Detection

作　　者：姜丽 梁峻毅 王玉彤 鲍林春 吴韵 郑玉国 夏光平 张小兰 张仟春 JIANG Li;LIANG Jun-yi;WANG Yu-tong;BAO Lin-chun;WU Yun;ZHENG Yu-guo;XIA Guang-ping;ZHANG Xiao-lan;ZHANG Qian-chun(School of Biology and Chemistry,Xingyi Normal University for Nationalities,Xingyi 562400,China;Key Laboratory of Chemical Synthesis and Environmental Pollution Control-Remediation Technology of Guizhou Province,Xingyi 562400,China;Clinical Laboratory,Qian Xi Nan Peoples Hospital,Xingyi 562400,China)

机构地区：[1]兴义民族师范学院生物与化学学院,贵州兴义562400 [2]贵州省化学合成及环境污染控制和修复技术特色重点实验室,贵州兴义562400 [3]黔西南州人民医院检验科,贵州兴义562400

出　　处：《分析测试学报》2020年第9期1065-1072,共8页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21505115);贵州省普通高等学校科技拔尖人才支持计划(黔教合KY字[2018]078);贵州省教育质量提升项目(2017014);贵州省教育厅青年科技人才成长项目(黔教合KY字[2019]222);黔西南州科学技术局项目(2019-2-53);功能材料开发及应用大学生创新实践基地(xysydcjd03)。

摘　　要：以L-瓜氨酸和尿素为前驱体,采用水热法制备了经济、低毒且具有强荧光性能的氮掺杂碳量子点(N-CQDs),并采用透射电子显微镜(TEM)、傅立叶变换红外光谱(FT-IR)、X射线光子能谱(XPS)等表征了N-CQDs的性能。结果表明,该N-CQDs的平均粒径为2.8 nm,量子产率为39.2%,具有良好的水溶性、稳定性和生物相容性,可用于HepG2细胞成像。此外,基于邻硝基苯酚(o-NP)对N-CQDs的荧光猝灭现象,构建了一种用于测定o-NP的高选择性、高灵敏的荧光探针。在o-NP浓度0.0833~12.0μmol/L范围内,N-CQDs荧光猝灭程度与o-NP浓度呈现良好的线性关系(r^2=0.998),检出限(S/N=3)为25.2 nmol/L。该方法已成功用于贵州省万峰湖和顶效河水样中o-NP的检测。A kind of nitrogen-doped carbon quantum dots(N-CQDs),with economy,low toxicity and even strong fluorescence performance,were successfully synthesized by hydrothermal method using L-citrulline and urea as precursors.The performance of N-CQDs was characterized by transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FT-IR)and X-ray photoelectron spectroscopy(XPS),etc.Results showed that the quantum yield of the N-CQDs reached up to 39.2%with an average particle size of 2.8 nm,which could be used for HepG2 cell imaging in view of their good water solubility,stability and biocompatibility.In addition,a fluorescence probe with high selectivity and sensitivity was proposed for the detection of o-nitrophenol(o-NP)based on an experimental phenomenon that the fluorescence of the CQDs could be specifically quenched by o-NP.Results showed that there was a good linear relationship for o-NP in the range of 0.0833-12.0μmol/L with a correlation coefficient(r^2)of 0.998 and a detection limit(LOD)of 25.2 nmol/L(S/N=3).The proposed method was successfully applied in the detection of o-NP in real water samples from Wanfeng Lake and Dingxiao River in Guizhou province.

关 键 词：氮掺杂碳量子点 细胞成像 水热法 邻硝基苯酚(o-NP) 水体 荧光猝灭 

分 类 号：O657.3[理学—分析化学] X131.2[理学—化学]