二郎山亚高山公路边坡土壤氮素与细菌群落组成的相关性研究  

Correlation between Soil Nitrogen and Bacterial Community Composition in Some Subalpine Highway Slopes

作　　者：宋伯翰 张宇冲 张月华 李伟[3] SONG Bo-han;ZHANG Yu-chong;ZHANG Yue-hua;LI Wei(College of Life Science,Sichuan Normal University,Chengdu 610101,China;Sinogra in Chengdu Storage Research Institute CO,LTD.Chengdu 610091,China;Key Laboratory of Mountain Surface Processes and Ecological Regulation,Institute of Mountain Hazards and Environment,Chinese Academy of Sciences and Ministry of Water Resources,Chengdu 610041,China)

机构地区：[1]四川师范大学生命科学学院,四川成都610101 [2]中储粮成都储藏研究院有限公司,四川成都610091 [3]中国科学院水利部成都山地灾害与环境研究所山地表生过程与生态调控重点实验室,四川成都610041

出　　处：《土壤通报》2020年第3期647-655,共9页Chinese Journal of Soil Science

基　　金：国家重点研发计划课题(2017YFC0504903)资助。

摘　　要：本文测定不同生长季节(3月、6月、10月)二郎山亚高山公路边坡的土壤氮素含量、微生物群落组成,探讨了两者间的相关性,旨在为该区域受损公路边坡植被恢复提供理论依据。结果表明:(1)与3月相比,4个样点土壤全氮含量呈现6月增加、10月减少;其中:铵态氮含量6月减少、10月增加的变化趋势;(2)3月、6月、10月各样点土壤细菌群落物种丰度指数的变化幅度分别为3530~3745、5018~6030、4121~5504,其中变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)为该区域公路边坡土壤中门分类水平下的优势菌群;马赛菌属(Massilia)、不动杆菌属(Acinetobacter)、假单胞菌属(Pseudomonas)和黄杆菌属(Flavobacterium)是该区域属分类水平下的优势菌群;(3)相关性分析结果表明,绿弯菌门(Chloroflexi)细菌丰富度与土壤总氮含量显著(P<0.05)正相关,与铵态氮含量极显著(P<0.01)负相关,厚壁菌门(Firmicutes)细菌丰富度与铵态氮含量极显著(P<0.01)正相关。Soil nitrogen(N)content,microbial community composition and their correlations were investigated in the highway slopes of the subalpine area of the Erlang Mountain at different growing seasons(March,June and October).The results showed that:(1)Compared with March,the total soil N content in four sampling points increased in June and decreased in October,while the content of soil ammonium N(NH4+-N)decreased in June and increased in October;(2)The Chao 1 index of soil bacterial community in each sampling point changed at the range of 3530-3745,5018-6030 and 4121-5504 in March,June and October,respectively.Proteobacteria,Bacteriodetes,Firmicutes,Acidobacteria and Actinobacteriawerethe dominant bacterial community at phylum level.Massilia,Acinetobacter,Pseudomonas and Flarobacteriumwere the dominant bacterial communityat genus level;(3)The richness of Chloroflexi was significantly positively related with total N content and extremely significantly negatively related with NH4+-N,and that of Firmicutes was extremely significantly positively correlated with NH4+-N.These research results were of great significance for the vegetation restoration in the damaged highway slopes in this region.

关 键 词：二郎山 土壤氨化作用 细菌群落组成 植被恢复 

分 类 号：S151.9[农业科学—土壤学]