新型冠状病毒肺炎住院和出院后不同标本中病毒核酸的检测  

Detection of 2019-nCoV nucleic acid in different specimens from confirmed COVID-19 cases during hospitalization and after discharge

作　　者：薛泽润 尤海菲 栾阳[1] 陈海龙[1] 杜全丽[1] 韩月雯 樊宁秀 杨玉捷 邓杨妮 李凯欣 党丽云 马超锋[1] 吴瑞[1] Xue Zerun;You Haifei;Luan Yang;Chen Hailong;Du Quanli;Han Yuewen;Fan Ningxiu;Yang Yujie;Deng Yangni;Li Kaixin;Dang Liyun;Ma Chaofeng;Wu Rui Viral(Diseases Laboratory,Xi′an Municipal Center for Disease Control and Prevention,Xi′an 710054,China;Institute of AIDS/STD Control and Prevention,Shaanxi Provincial Center for Disease Control and Prevention,Xi′an 710054,China;Department of Immunization Program,Shiquan County Center for Disease Control and Prevention,Ankang 725200,China;Tuberculosis Internal Medicine Department,Xi′an Chest Hospital,Xi′an 710100,China)

机构地区：[1]西安市疾病预防控制中心病毒科,710054 [2]陕西省疾病预防控制中心艾滋病预防控制所,西安710054 [3]石泉县疾病预防控制中心免疫规划科,安康725200 [4]西安市胸科医院结核内科,710100

出　　处：《中华微生物学和免疫学杂志》2020年第8期569-573,共5页Chinese Journal of Microbiology and Immunology

基　　金：西安市新型冠状病毒肺炎疫情应急防治科技专项(20200003YX003-1,20200001YX001-2)。

摘　　要：目的分析COVID-19确诊病例住院期间和出院后不同类型标本中病毒核酸的检出情况。方法调查定点医院收治的COVID-19确诊病例,住院期间采集其鼻咽拭子、粪便、尿液和血液标本,出院后进行随访,采集其鼻咽拭子和粪便标本,采用实时荧光RT-PCR方法进行核酸检测。结果共调查25例COVID-19确诊病例,在住院期间采集其鼻咽拭子、粪便、尿液和血液标本各25份,共100份标本,经检测鼻咽拭子均为病毒核酸阳性,住院期间9例患者粪便中检出核酸阳性,阳性率为36.00%(9/25),尿液和血液未检出病毒核酸。25例患者出院后进行随访,在第7天和第14天采集其鼻咽拭子和粪便标本各25份,第7天2例患者检出鼻咽拭子核酸阳性,复阳率为8.00%(2/25),粪便中未检出病毒核酸,出院后第14天鼻咽拭子和粪便中均未检出病毒核酸。结论COVID-19确诊病例住院治疗期间粪便中可检出病毒核酸,出院后部分患者鼻咽拭子病毒核酸复阳。Objective To investigate the positive rates of 2019-nCoV nucleic acid in different specimens from confirmed COVID-19 cases during hospitalization and after discharge.Methods Patients with confirmed COVID-19 were enrolled from designated hospitals.Nasal swabs,throat swabs,and specimens of stool,urine and blood were collected during hospitalization.After the patients were discharged,nasal swabs,throat swabs and stool specimens were collected during follow-up.Real-time RT-PCR was used to detect 2019-nCoV nucleic acid.Results This study involved 25 confirmed COVID-19 cases.During hospitalization,all patients tested positive in both nasal and throat swab 2019-nCoV nucleic acid tests,and nine of them(36.00%)were positive in stool specimen test.Urine and blood specimen test results were all negative.Nasal swabs,throat swabs and stool specimens were collected from each patient 7 d and 14 d after discharge.Two patients(8.00%)tested positive for 2019-nCoV nucleic acid again in nasal and throat swab tests on 7 d,while all stool specimen tests were negative.No 2019-nCoV nucleic acid was detected in nasal swabs,throat swabs or stool samples on 14 d.Conclusions 2019-nCoV nucleic acid was detected in stool samples of confirmed COVID-19 cases during hospitalization.Nasal and throat swab nucleic acid tests turned positive again in some patients after discharge.

关 键 词：新型冠状病毒肺炎 新型冠状病毒 粪便 出院病例 

分 类 号：R563.1[医药卫生—呼吸系统] R440[医药卫生—内科学]