姜黄素通过激活miR-192-5p/PI3K/Akt信号通路抑制肾母细胞瘤增殖并诱导细胞凋亡  

Curcumin inhibits the proliferation and induces the apoptosis of nephroblastoma through activating miR-192-5p/PI3K/Akt signaling pathway

作　　者：苌俊明 李德亮[1] 朱萍[1] 程雪莲[1] 别静洋[1] 张伟业 包逢源 席悦 李营营 Chang Junming;Li Deliang;Zhu Ping;Cheng Xuelian;Bie Jingyang;Zhang Weiye;Bao Fengyuan;Xi Yue;Li Yingying(Neonatal Intensive Care Center,Nanyang Central Hospital,Nanyang 473000,China;Department of Gastroenterology No.2 Ward,Nanyang Central Hospital,Nanyang 473000,China)

机构地区：[1]南阳市中心医院新生儿重症监护病房,473000 [2]南阳市中心医院消化科二病区,473000

出　　处：《中华微生物学和免疫学杂志》2020年第8期622-627,共6页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究姜黄素通过激活miR-192-5p/PI3K/Akt信号通路抑制肾母细胞瘤增殖并诱导细胞凋亡。方法采用CCK-8法探讨姜黄素对肾母细胞瘤SK-NEP-1细胞增殖的影响,并探究其合适的作用浓度;V-FITC/PI检测SK-NEP-1细胞的凋亡率;荧光素酶报告试验验证miR-192-5p和PI3K的结合活性;RT-PCR检测miR-192-5p mRNA的表达水平;Western blot检测PI3K和Akt的蛋白质表达水平。结果姜黄素能够显著抑制SK-NEP-1细胞的增殖,并诱导细胞凋亡,且呈剂量依赖性。RT-PCR检测结果表明姜黄素能够显著提高miR-192-5p的表达。此外,miR-192-5p显著抑制细胞的增殖并诱导其凋亡,且能够增强姜黄素对SK-NEP-1细胞的作用。荧光素酶报告试验结果表明miR-192-5p能够靶向结合PI3K,Western blot结果表明姜黄素可通过介导miR-192-5p的表达抑制PI3K和Akt的蛋白质表达水平。结论姜黄素能够抑制肾母细胞瘤细胞的增殖并诱导其凋亡,其作用机制是通过介导miR-192-5p的表达并进一步抑制下游PI3K/Akt信号通路来实现的。Objective To study whether curcumin inhibits the proliferation and promotes the apoptosis of nephroblastoma through activating the miR-192-5p/PI3K/Akt signaling pathway.Methods CCK-8 assay was used to investigate the effects of curcumin on the proliferation of nephroblastoma SK-NEP-1 cells and the appropriate concentration.The apoptosis rate of SK-NEP-1 cells was detected by V-FITC/PI.Luciferase reporter assay was used to verify the binding activity between miR-192-5p and PI3K.RT-PCR was performed to detect the expression of miR-192-5p at mRNA level.Western blot was used to detect the expression of PI3K and Akt at protein level.Results Curcumin could significantly inhibit the proliferation of SK-NEP-1 cells and induce cell apoptosis in a dose-dependent manner.RT-PCR results showed that curcumin could significantly increase the expression of miR-192-5p.In addition,miR-192-5p significantly inhibited cell proliferation,induced cell apoptosis,and enhanced the effects of curcumin on the proliferation and apoptosis of SK-NEP-1 cells.Luciferase reporter assay suggested that miR-192-5p could bind to PI3K.Western blot results showed that curcumin down-regulated the expression of PI3K and Akt at protein level by mediating the expression of miR-192-5p.Conclusions Curcumin could inhibit the proliferation and induce the apoptosis of nephroblastoma cells through mediating the expression of miR-192-5p and further inhibiting the downstream PI3K/Akt signaling pathway.

关 键 词：姜黄素 肾母细胞瘤 miR-192-5p PI3K/AKT信号通路 

分 类 号：R285.5[医药卫生—中药学]