浓缩生长因子修复兔髁突全层软骨损伤  

Concentrated growth factors in the repair of a full-thickness condylar cartilage defect in a rabbit

作　　者：吴训 孟娟红[1] 张建运 王亮 Wu Xun;Meng Juanhong;Zhang Jianyun;Wang Liang(Department of Oral and Maxillofacial Surgery,Peking University School and Hospital of Stomatology&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology,Beijing 100081,China;Department of Oral Pathology,Peking University School and Hospital of Stomatology&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology,Beijing 100081,China)

机构地区：[1]北京大学口腔医学院·口腔医院,口腔颌面外科,北京市100081 [2]北京大学口腔医学院·口腔医院,口腔病理科,口腔数字化医疗技术和材料国家工程实验室,口腔数字医学北京市重点实验室,北京市100081

出　　处：《中国组织工程研究》2021年第8期1166-1171,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:浓缩生长因子对组织修复有促进作用,目前尚缺乏其对髁突软骨修复影响的研究。目的:研究浓缩生长因子对兔颞下颌关节髁突全层软骨损伤修复的影响。方法:采集兔静脉血制备浓缩生长因子。建立兔双侧髁突穿透软骨下骨皮质的全层软骨损伤模型,实验侧损伤区充填浓缩生长因子,对照侧自然愈合。分别于术后2,6,12周取材并进行组织形态学观察;采用改良Pineda软骨修复评分评价软骨修复程度;测定兔浓缩生长因子中部分生长因子释放速率随时间的变化。实验已通过北京大学医学部动物伦理委员会审核批准,批准号为LA201809。结果与结论:①术后2周实验侧和对照侧缺损内可见纤维样及软骨样组织充填;实验侧甲苯胺蓝染色较均匀,对照侧细胞间纤维样组织穿插,甲苯胺蓝染色不均匀;术后6,12周实验侧仍可见软骨样组织修复,对照侧多见纤维样组织增生覆盖,甲苯胺蓝染色不着色;②改良Pineda软骨修复评分:术后6周及12周实验侧修复的软骨样组织形态评分优于对照侧;实验侧12周较2周修复软骨样组织形态差异不明显(P>0.05),而对照侧12周较2周修复组织软骨成分减少,软骨修复评分增高,差异有显著性意义(P<0.05);③ELISA实验结果表明兔浓缩生长因子中胰岛素样生长因子1、转化生长因子β1、碱性成纤维细胞生长因子、血管内皮细胞生长因子可持续释放14 d以上,平均释放速率随时间递减;④以上结果表明,浓缩生长因子对兔髁突全层软骨损伤的早期软骨修复有一定促进作用。BACKGROUND:Concentrated growth factors can promote the repair of tissue injuries.Its effect on the repair of condylar cartilage injuries is rarely documented.OBJECTIVE:To investigate the effect of concentrated growth factors on the repair of full-thickness condylar cartilage defects in rabbits.METHODS:Concentrated growth factors were prepared by collecting the venous blood of rabbits.A full-thickness cartilage defect penetrating the subchondral cortex was created at both sides of condyle in rabbits.The experimental side was filled with concentrated growth factors,and the control side healed naturally.The histomorphology was examined at 2,6 and 12 weeks postoperatively.The degree of cartilage repair was evaluated by the modified Pineda cartilage repair scale.The release rate of concentrated growth factors was measured at different observational times.The study protocol was approved by the Experimental Animal Ethics Committee of Peking University Health Science Center(approval No.LA201809).RESULTS AND CONCLUSION:Fillings of fibrous and cartilage-like tissue in the defect were observed on both of the experimental and the control sides at 2 weeks postoperatively.Toluidine blue was stained homogeneously in the experimental side.Intercellular fibrous tissues with interpenetrating and heterogeneous toluidine blue staining appeared in the control side.At 6 and 12 weeks postoperatively,the repaired cartilage was identified in the experimental side.On the contrary,fibroid tissue hyperplasia was found in the control side,where toluidine blue staining showed no heterochromatin.Based on the modified Pineda cartilage repair score,the mean value of the total score in the experimental side was better than that in the control side at 6 and 12 weeks postoperatively.The difference in the mean value of the total score(P<0.05)was significant between 2 and 12 weeks in the control side,but there was no significant difference in the experimental side(P>0.05).ELISA tests showed that insulin-like growth factor 1,transforming growth factor

关 键 词：浓缩生长因子 颞下颌关节 髁突 软骨 修复 实验 兔 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]