CREB在反复喘息患儿中的表达及对ORMDL3基因表达的影响  

Expression of CREB in children with recurrent wheezing and its effect on ORMDL3 gene expression

作　　者：金蕊[1] 张振兴[1] 朱亮华[1] 庄丽丽[1] 陈筱青[1] JIN Rui;ZHANG Zhen-Xing;ZHU Liang-Hua;ZHUANG Li-Li;CHEN Xiao-Qing(Department of Pediatrics,First Affiliated Hospital,Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院儿科,江苏南京210029

出　　处：《中国当代儿科杂志》2020年第9期980-983,共4页Chinese Journal of Contemporary Pediatrics

基　　金：江苏省科教强卫工程青年医学人才项目(QNRC2016615);江苏省妇幼健康重点人才项目(FRC201736)。

摘　　要：目的探讨环磷腺苷效应元件结合蛋白(CREB)在3岁以下反复喘息患儿中的表达水平及其对血清类黏蛋白1样蛋白3基因(ORMDL3)表达水平的影响。方法选取2017年6月至2019年6月就诊的3岁以下反复喘息儿童36例为病例组,另选取行健康体检的24例儿童为健康对照组,使用荧光定量PCR法检测外周血CREB的表达水平。培养人支气管上皮细胞(BEAS-2B),使用双荧光素酶报告基因和荧光定量PCR法检测过表达和siRNA干扰CREB对BEAS-2B细胞ORMDL3基因启动子活性及m RNA表达水平的影响。结果病例组CREB表达水平高于健康对照组(P<0.001)。在BEAS-2B细胞中,过表达CREB能上调ORMDL3基因的启动子活性及m RNA表达水平(P<0.05);siRNA干扰CREB的表达后ORMDL3基因的启动子活性及m RNA表达水平均降低(P<0.05)。结论反复喘息患儿CREB表达增加,其可能通过调控ORMDL3基因的表达参与了反复喘息的发病。Objective To study the expression level of cAMP response element-binding protein(CREB)in children with recurrent wheezing under three years of age and its effect on the expression of the serum orosomucoid 1-like protein 3(ORMDL3)gene.Methods Thirty-six children with recurrent wheezing under three years of age who visited the hospital from June 2017 to June 2019 were selected as the recurrent wheezing group.Twenty-four healthy children from physical examination were selected as the control group.The CREB expression level in peripheral blood was measured by quantitative real-time PCR.Human bronchial epithelial cells(BEAS-2 B)were cultured,and dualluciferase reporter assay and quantitative real-time PCR were used to investigate the effects of overexpression and siRNA interference of CREB on the promoter activity and mRNA expression of the ORMDL3 gene in the BEAS-2 B cells.Results The expression level of CREB in the recurrent wheezing group was significantly higher than that in the control group(P<0.001).In BEAS-2 B cells,overexpression of CREB significantly up-regulated the promoter activity and mRNA expression of the ORMDL3 gene(P<0.05),while siRNA interference of CREB significantly reduced the promoter activity and mRNA expression of the ORMDL3 gene(P<0.05).Conclusions The expression of CREB is increased in children with recurrent wheezing,and CREB may be involved in the pathogenesis of recurrent wheezing by regulating expression of the ORMDL3 gene.

关 键 词：环磷腺苷效应元件结合蛋白 ORMDL3 反复喘息 儿童 

分 类 号：R725.6[医药卫生—儿科]