在小麦和叶锈菌互作过程中TCTP的表达特征  

Expression Characteristic of Translationally Controlled Tumor Protein during the Interaction between Wheat and Puccinia Triticina

作　　者：路兴通 麻楠 孙天杰[1] 郭云杉 常亭亭 王冬梅[1] LU Xing-tong;MA Nan;SUN Tian-jie;GUO Yun-shan;CHANG Ting-ting;WANG Dong-mei(Hebei Agricultural University,College of Life Sciences/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,B aoding 071001)

机构地区：[1]河北农业大学生命科学学院/华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,保定071001

出　　处：《植物遗传资源学报》2020年第5期1230-1236,共7页Journal of Plant Genetic Resources

基　　金：国家自然科学基金项目(31171472,31871548);高等学校博士学科点专项科研基金资助课题(优先发展领域)(20111302130001);河北省应用基础研究计划重点基础研究项目(12967149D)。

摘　　要：翻译控制肿瘤蛋白是调节植物生长发育、参与植物抵抗逆境胁迫的重要调节因子。本研究以叶锈菌生理小种260与4种含有不同抗叶锈基因的近等基因系分别组成亲和程度不同的组合为对象,采用RT-qPCR和Western Blotting方法,从转录和翻译两个水平探讨TCTP在小麦与叶锈菌互作过程中的表达模式。研究结果表明,无论是转录水平还是翻译水平上,TCTP在不亲和组合中均表现为表达量显著上调,但在不同的Lr基因背景下其表达模式有所不同;而在亲和组合中并未表现出明显的变化趋势。基于TCTP mRNA和蛋白水平的结果,发现在小麦与叶锈菌互作体系中TCTP在转录水平和翻译水平均受到调控,推测其在小麦抵抗叶锈菌侵染的防卫反应中可能发挥正调控作用,这将为进一步研究TCTP的功能及其作用机制奠定了基础。Translationally controlled tumor protein(TCTP) is an important regulator involved in plant growth and development as well as responses to biotic and abiotic stresses. By taking use of Puccinia triticina physiological race 260 and four wheat near-isogenic lines(NILs) that form combinations with different affinity degrees,we investigated the expression of TCTP at transcription and translational levels using RT-qPCR and Western Blotting,respectively. The transcriptional level of TCTP was significantly up-regulated in the incompatible combination,but this was diverse in NILs with Lr gene. No obvious change trend in the affinity combination was observed. Gained from the results of TCTP mRNA and protein levels,the expression of TCTP was found to be modified at both transcription and translation level during the interaction between wheat and Puccinia triticina. It is speculated that it may play a positive role in the defense response of wheat against Puccinia triticina infection. This will lay the foundation for further research on the function and mechanism of TCTP.

关 键 词：TCTP 小麦 叶锈菌 表达分析 

分 类 号：S435.121.43[农业科学—农业昆虫与害虫防治]