利用高代回交导入群体定位大豆品质性状QTL  

QTL Mapping of Quality Traits in Soybean with Advanced Backcross Introgression Population

作　　者：任丙新 韩粉霞[1] 杨华[1] REN Bing-xin;HAN Fen-xia;YANG Hua(Institute of Crop Science,Chinese Academy of Agricultural Sciences/Key Laboratory of Soybean Biology(Beijing),Ministry of Agriculture,Beijing 100081)

机构地区：[1]中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室,北京100081

出　　处：《植物遗传资源学报》2020年第5期1255-1262,共8页Journal of Plant Genetic Resources

基　　金：国家转基因生物新品种培育重大专项(2016ZX08004-004);国家重点研发计划项目(2017YFD0101400);中国农业科学院科技创新工程项目。

摘　　要：通过对大豆品质性状的QTL定位,可以为大豆分子育种提供理论依据。本研究以中黄13(轮回亲本)×东山69(供体亲本)的142个家系的高代回交导入系群体BC2F7,利用具有多态性的113对SSR引物构建了一张涵盖除B2之外的19个大豆连锁群、总长度为1630.95 cM、平均遗传距离为14.43 cM的遗传连锁图谱。利用近红外光谱分析法(NIR)、气相色谱技术(GC)分析群体蛋白质、脂肪及5种脂肪酸主要组分含量;采用ICIM-ADD作图法定位QTL。两年共定位到34个QTL,其中与蛋白质、脂肪、硬脂酸、棕榈酸、亚油酸、油酸和亚麻酸相关的QTL分别为5、2、11、6、3、4、3个,Sat003~Sat147两年均被检测出与油酸含量呈负相关,5个区间与2个性状含量相关,Satt523~Sat405与棕榈酸、蛋白质相关,Staga001~Satt658、Satt263~Satt651与油酸、硬脂酸相关,Satt228~Satt525与亚油酸、蛋白质相关,Satt658~Satt433与亚麻酸、硬脂酸相关;总体而言,定位结果在一定程度上阐明了品质性状的相关性,对大豆分子育种中优良品种的选育具有参考意义。QTL mapping of soybean quality traits can provide a theoretical basis for molecular hybridization breeding. In this study,113 genetic pairs of SSR primers with polymorphism were used to construct a genetic linkage map with a total length of 1630.95 cM and an average genetic distance of 14.43 cM,covering 19 soybean linkage groups(except B2)of 142 lines of advanced backcross introgression population BC2 F7 derived from soybean ‘Zhonghuang 13’(recurrent parent)בDongshan 69’(donor parent). Near-infrared spectroscopy(NIR)and gas chromatography(GC)were used to determine the contents of protein,oiland five major fatty acids in soybean. A total of 34 QTLs were identified using ICIM-ADD in two years. The number of mapped QTL were 5,2,11,6,3,4,and 3 for protein,oil,stearic acid,palmitic acid,linoleic acid,oleic acid,and linolenic acid,respectively. Sat003-Sat147 were negatively correlated with oleic acid in the two years. Satt523-Sat405 were correlated with palmitic acid and protein. Staga001-Satt658 and Satt263-Satt651 were correlated with oleic acid and stearic acid. Satt228-Satt525 were correlated with linoleic acid and protein. Satt658-Satt433 were correlated with linolenic acid and stearic acid. The mapping results have clarified the correlation of quality traits to a certain extent,and have reference significance for the selection of excellent varieties in soybean molecular breeding.

关 键 词：大豆 高代回交群体 品质性状 QTL 定位 

分 类 号：S565.1[农业科学—作物学]