外源蔗糖对离体条件下换锦花小鳞茎发生的影响  

Effects of Exogenous Sucrose on Bulblet Formation of Lycoris sprengeri in vitro

作　　者：吕学思 张栋[1] 闵芮涵 李世琦 李铮 任梓铭[1] 夏宜平[1] Lü Xuesi;ZHANG Dong;MIN Ruihan;LI Shiqi;LI Zheng;REN Ziming;XIA Yiping(Genomics and Genetic Engineering Laboratory of Ornamental Plants,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学农业与生物技术学院园林研究所,观赏植物基因工程实验室,杭州310058

出　　处：《园艺学报》2020年第8期1475-1489,共15页Acta Horticulturae Sinica

基　　金：浙江省“十三五”农业(林木)新品种选育重大科技专项(2016C02056-13);国家自然科学基金项目(31772337)。

摘　　要：从形态学、组织细胞学、生理生化水平、分子水平等多层面探究外源蔗糖浓度对换锦花(Lycori sprengeri)离体小鳞茎发生的影响。结果表明,MS培养基中外源蔗糖缺失时,离体换锦花鳞茎无法产生小鳞茎,蔗糖浓度为30或60 g·L^-1时小鳞茎正常发生,但对小鳞茎发生数量影响不大。鳞片内的蔗糖及可溶性糖含量在腋芽形成前不断积累,且鳞片基部淀粉粒的积累为此处小鳞茎的发生提供物质基础。蔗糖促进了茉莉酸甲酯(MeJA)在植物体内的积累,抑制了LsWIP1、LsERS2和LsEIN2基因表达量的异常上升,表明蔗糖可能作为信号分子启动了相关损伤保护机制,并促进了小鳞茎的发生。Lycoris sprengeri was used to study the effects of exogenous sucrose concentrations on bulblet formation in vitro,from morphological,histocytological,physiological,biochemical and molecular levels.The results showed that the presence of exogenous sucrose is a necessary condition for the formation of bulblets in vitro,while sucrose concentration(30 g·L^-1 or 60 g·L^-1)had little effect on the number of bulblets.Acting as both carbon and energy source,exogenous sucrose was a key factor for bulblet formation.When exogenous sucrose was present,sucrose and soluble sugar accumulated before the formation of axillary buds,and the accumulation of starch granules at the base of the scale provided a material basis for bulblet formation here.In addition,the presence of sucrose promoted the accumulation of Me JA in plants and inhibited the abnormal increase in the expression of Ls WIP1,Ls ERS2 and Ls EIN2 genes levels,which may mean that sucrose initiated a damage protection mechanism as a signaling molecule and promoted the formation of bulblet.

关 键 词：换锦花 离体培养 蔗糖 小鳞茎发生 

分 类 号：S682[农业科学—观赏园艺]