TG2对大鼠鼻粘膜间充质干细胞体外的影响  

Effect of tissue transglutaminase 2 on rat nasal mucosal mesenchymal stem cells in vitro

作　　者：阙云端 卞璐 吕德民 史文涛 崔学文 王东明 张志坚[2] QUE Yun-duan;BIAN Lu;LV De-min;SHI Wen-tao;CUI Xue-wen;WANG Dong-ming;ZHANG Zhi-jian(Department of Orthopaedics,Nanjing Gaochun People's Hospital,Nanjing 211300,China;School of Medicine,Jiangsu University,Zhenjiang 212000,China)

机构地区：[1]南京市高淳人民医院骨科,南京211300 [2]江苏大学医学院,江苏镇江212000

出　　处：《中国矫形外科杂志》2020年第17期1591-1595,共5页Orthopedic Journal of China

基　　金：国家自然科学基金地区科学基金资助项目(编号:81571830)。

摘　　要：[目的]探讨组织型谷胺酰氨转胺酶2(TG2)对大鼠鼻粘膜来源的间充质干细胞体外增殖以及定向成骨分化的影响。[方法]体外分离培养大鼠鼻粘膜间充质干细胞,采用MTT和免疫荧光法检测不同浓度的TG2对大鼠鼻粘膜间充质干细胞增殖能力的影响。通过茜素红染色、碱性磷酸酶染色、蛋白质印迹法和免疫荧光观察TG2对大鼠鼻粘膜间充质干细胞体外成骨分化能力的影响。[结果]谷胺酰氨转胺酶2能促进鼻粘膜间充质干细胞增殖(P<0.05),并能提高ALP活性(P<0.05),同时增强矿化钙结节形成能力,并对成骨相关标记蛋白COL I和OCN的表达具有上调作用。[结论]谷胺酰氨转胺酶2可提高大鼠鼻粘膜来源的间充质干细胞的增殖能力以及其向成骨细胞分化的潜能。[Objective]To explore the effects of tissue transglutaminase 2(TG2)on proliferation and osteogenic differentiation of rat ectomesenchymal stem cells(EMSCs)obtained from nasal mucosa in vitro.[Methods]EMSCs isolated form rat nasal mucosa were cultured in vitro with different concentrations of TG2.The proliferation of EMSCs were detected by MTT assay.In addition,the osteogenic differentiations of the cells were assessed by alizarin red staining alkaline phosphatase staining,Western blotting and immunofluorescence.[Results]The TG2 promoted cell proliferation(P<0.05),enhancing mineralized nodules(P<0.05),and increased ALP activity of rat EMSCs.In addition,the TG2 also upregulated the cell s expression of osteogenesisrelated marker proteins COL I and OC in vitro.[Conclusion]The tissue transglutaminase 2 does enhance proliferation and osteogenic differentiation of rat ectomesenchymal stem cells obtained from nasal mucosa.

关 键 词：组织型谷氨酰胺转氨酶(TG-2) 外胚间充质干细胞 细胞增殖 成骨分化 

分 类 号：R687.4[医药卫生—骨科学]