脊髓灰质炎病毒假病毒中和试验测定血清中和抗体滴度的研究  

Analyses of the Neutralizing-Antibody Titer in Serum Carrying the Poliovirus Pseudovirus for a Poliovirus Neutralization Test

作　　者：吴雅楠[1] 朱文勇[1] 周健[1] 刘泽[1] 马磊[1] 高菁霞[1] 廖国阳[1] WU Yanan;ZHU Wenyong;ZHOU Jian;LIU Ze;MA Lei;GAO Jingxia;LIAO Guoyang(Institute of Medical Biology,Chinese Academy of Medical Science and Peking Union Medical College,KunMing 650000,China)

机构地区：[1]中国医学科学院,北京协和医学院,医学生物学研究所,昆明650000

出　　处：《病毒学报》2020年第5期802-808,共7页Chinese Journal of Virology

基　　金：中国医学科学院医学科技创新项目(项目号:2016-I2M-3-026),题目:疫苗关键技术创新团队;云南省重点研发项目(项目号:2017IB008),题目:替代活病毒中和试验评价脊髓灰质炎疫苗免疫效果和监测人体免疫保护力关键技术国际合作研究;云南省科技计划项目(项目号:2017FB041),题目:脊髓灰质炎疫苗衍生病毒基因重组变异与疫苗保护性的相关性研究;云南省重大科技计划项目(项目号:2017ZF008),题目:Sabin株脊髓灰质炎灭活疫苗提质增效新工艺研究。

摘　　要：脊髓灰质炎病毒(poliovirus,PV)的持续监测在全球消灭脊髓灰质炎的最后阶段起着至关重要的作用,对易感人群的脊髓灰质炎中和抗体的监测,是疫苗强化免疫的重要数据支持。但是伴随着脊灰活病毒的大范围封存计划的实施,传统的病毒中和试验将难以开展,为此本研究成功构建了SabinⅡ型PV假病毒,并将其运用到人群血清样本的中和试验进行验证。研究发现,PV假病毒具有型特异性,具有与脊灰病毒相似的电镜形态。所以本研究制备的脊灰假病毒能够满足人群血清中和抗体水平的检测要求,值得推广运用。At the final stageend game of global polio eradication,monitoring of the neutralizing antibody titer against poliomyelitis plays an important part in vaccine immunization for susceptible populations. Conventional neutralization tests cannot be carried out for fear of potential retention of the infectious poliovirus(PV). We constructed a Sabin II PV pseudovirus. Then,we used a PV neutralization test with a type 2 pseudovirus to determine the titer of neutralizing antibody against PV-2 in human serum samples. The PV pseudovirus had a type specificity for detection of neutralizing antibody,and its morphology(according to electron microscopy)was similar to that of the PV. Therefore,the PV pseudovirus employed in this study can meet the requirements for detection of the neutralizing-antibody level in serum in the population,and merits extensive use.

关 键 词：脊髓灰质炎病毒 假病毒 中和试验 中和抗体滴度 

分 类 号：R563.1[医药卫生—呼吸系统]