过氧化氢酶基因单核苷酸多态性与职业人群噪声性听力损失易感性  

Association between single nucleotides polymorphism of catalase gene and susceptibility to noise-induced hearing loss in occupational population

作　　者：李坛 陈国顺 焦洁[1] 周文慧 吴辉 谷桂珍 李艳红 许雪春 张焕玲 郑玉新[4] 余善法[1,5] Li Tan;Chen Guoshun;Jiao Jie;Zhou Wenhui;Wu Hui;Gu Guizhen;Li Yanhong;Xu Xuechun;Zhang Huanling;Zheng Yuxin;Yu Shanfa(College of Public Health,Zhengzhou University,Zhengzhou 450001,China;Wugang Institute for Occupational Health,Pingdingshan 462500,China;Henan Provincial Institute for Occupational Health,Zhengzhou 450000,China;College of Public Health,Qingdao University,Qingdao 266071,China;Henan Medical College,Zhengzhou 451191,China)

机构地区：[1]郑州大学公共卫生学院,郑州450001 [2]舞钢劳动卫生职业病防治研究所,平顶山462500 [3]河南省职业病防治研究院,郑州450000 [4]青岛大学公共卫生学院,青岛266071 [5]河南医学高等专科学校,郑州451191

出　　处：《卫生研究》2020年第5期716-723,共8页Journal of Hygiene Research

基　　金：国家自然科学基金(No.81372940,81872574);国家科技支撑计划(No.2014BAI12B03)。

摘　　要：目的探讨过氧化氢酶(catalase, CAT)基因单核苷酸多态性与职业噪声接触人群噪声性听力损失(noise-induced hearing loss, NIHL)易感性间的关系。方法基于2006—2015年对河南省某钢铁厂职业噪声接触工人的队列研究,采用1∶1匹配的巢式病例-对照研究方法,从队列中选出双耳高频平均听阈≥40 dB(HL),共286人,根据工种、性别相同,年龄相差不超过5岁且接触噪声工龄相差不超过2年进行匹配,在该队列中选出听力正常的对照组286人。用中高通量单核苷酸多态性分型检测技术(SNPscanTM法)检测CAT基因的8个单核苷酸位点的多态性,采用多因素条件Logistic回归法分析CAT基因8个单核苷酸多态性位点与NIHL易感性之间的关系。结果 CAT基因rs208679位点的显性模型[(GA+GG)/AA]下,携带GA或GG基因型的个体患NIHL的风险是携带AA基因型个体的1.431倍(95%CI 1.020~2.009),经检验P=0.038。结论 CAT基因rs208679位点突变型等位基因G可能为NIHL易感性的危险因素之一。OBJECTIVE To investigate the relationship between single nucleotides polymorphism of catalase(CAT) gene and susceptibility to noise-induced hearing loss(NIHL) in occupational noise exposed population. METHODS A case-control study of 1∶1 was conducted to select 286 workers with binaural high frequency average hearing threshold ≥40 dB(HL), from 2006 to 2015 in a cohort study of occupational noise exposure workers in Henan Province. According to the type of work, the age difference was not more than 5 years and the length of exposure to noise was not more than 2 years. The polymorphism of 8 single nucleotides in CAT gene was detected by medium SNPscanTM, and the relationship between 8 single nucleotides polymorphism of CAT gene and NIHL susceptibility was analyzed by multivariate conditional logistic regression. RESULTS Under the dominant model of rs208679 locus of CAT gene [(GA GG)/AA], the risk of NIHL in individuals carrying GA or GG genotype was 1.431 times higher than that in individuals carrying AA genotype(95%CI 1.020-2.009), and P=0.038. CONCLUSION G, a mutant at rs208679 site of CAT gene, may be one of the risk factors for NIHL susceptibility.

关 键 词：噪声性听力损失 职业性 过氧化氢酶基因 易感性 单核苷酸多态性 

分 类 号：R135.8[医药卫生—劳动卫生]