类志贺邻单胞菌感染杂交鲟肠道组织转录组分析  

Transcriptome Analysis of Hybrid Sturgeons Intestinal Infected with Plesiomonas shigelloides

作　　者：张明洋 曾茂芹 刘妍罕 张飘 杨霞 杨颖[1,2] 温贵兰 程振涛[1,2,3] 姜海波 文明 ZHANG Mingyang;ZENG Maoqin;LIU Yanhan;ZHANG Piao;YANG Xia;YANG Ying;WEN Guilan;CHENG Zhentao;JIANG Haibo;WEN Ming(College of Animal Science,Guizhou University,Guiyang 550025,China;Key Laboratory of Animal Epidemiology and Veterinary Politic Heath of Guizhou,Guiyang 550025,China;Research Center of Engineering Technology for Animal Biological Products of Guizhou,Guiyang 550025,China)

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州省动物疫病与兽医公共卫生重点实验室,贵阳550025 [3]贵州省动物生物制品工程技术研究中心,贵阳550025

出　　处：《中国畜牧兽医》2020年第9期2732-2740,共9页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省科技支撑计划项目(黔科合支撑[2016]2502号、黔科合支撑[2016]2511号、黔科合支撑[2017]2532号);贵州省科技平台及人才团队计划项目(黔科合平台人才[2018]5253号)。

摘　　要：为探究病原菌感染杂交鲟肠道转录组变化情况,本试验以常见病原菌类志贺邻单胞菌接种约360 d杂交鲟,于接种24 h后,采集杂交鲟肠道组织样本,提取组织总RNA,采用Illumina HiSeqTM 2000进行转录组测序,筛选杂交鲟肠道差异表达免疫应答基因并进行GO功能分类和KEGG信号通路分析,结果显示:与正常对照组比较,试验感染组杂交鲟肠道差异表达基因为13542个,其中上调基因为9774个,下调基因为3768个;GO分析发现,杂交鲟肠道差异表达基因显著性富集到生物学过程的主要有免疫系统过程、免疫效应过程、刺激反应等;显著性富集到分子功能的主要有DNA结合、信号受体活性、核酸转录因子活性等;显著性富集到细胞组分的主要是胞外区、胞外区组成部分、细胞膜等。KEGG分析发现,杂交鲟肠道差异表达基因参与的免疫信号通路有RIG-Ⅰ样受体信号通路、Toll样受体信号通路和细胞溶质DNA传感途径。这些研究结果为深入探究杂交鲟对肠道病原微生物感染的防御分子机制奠定了良好的基础。In order to explore the changes of intestinal transcriptome of sturgeons infected with pathogenic bacteria,the hybrid sturgeons about 360 d were infected by Plesiomonas shigelloides,and their intestinal tissues were collected after 24 h of inoculation and used for extraction of mRNA,and transcriptome sequencing was performed using high-throughput sequencing technology and analyzed for GO function classification and KEGG signal pathway.The results showed that there were 13542 of differentially expressed genes with 9774 of up-regulated genes and 3768 of down-regulated genes in the hybrid sturgeon intestinal comparing with the control group.GO analysis found that significantly enriched sturgeons gut differentially expressed genes were mainly involved in immune system process,immune effect process and stimulation response.Significant enrichment of molecular functions mainly included:DNA binding,signal receptor activity,nucleic acid transcription factor activity.The cell components were mainly extracellular,extracellular components,cell membrane.KEGG analysis showed that the immune signaling pathways involved in differentially expressed genes in sturgeons intestinal were RIG-Ⅰ-like receptor signaling pathway,Toll-like receptor signaling pathway and cytosolic DNA-sensing pathway.These results laid a good foundation for the defense molecular mechanism of the hybrid sturgeon against pathogenic infection.

关 键 词：杂交鲟 肠道组织 高通量测序 转录组 类志贺邻单胞菌 

分 类 号：S852.612[农业科学—基础兽医学]