3种动物专用抗菌药在鸡肠道沙门菌的流行病学临界值的建立  

Epidemiological Cut-off Values(ECOFFs)of Salmonella from Chicken Intestinal Tract for Three Animal-specific Antibiotics

作　　者：吴思莉 傅嘉莉 朱家杭 张岩 丁晓敏 蒋红霞[1] WU Sili;FU Jiali;ZHU Jiahang;ZHANG Yan;DING Xiaomin;JIANG Hongxia(National Veterinary Drug Safety Evaluation Laboratory,College of Veterinary Medicine,South China Agricultural University, Guangzhou 510642,China)

机构地区：[1]华南农业大学兽医学院,国家兽药安全评价实验室,广州510642

出　　处：《中国畜牧兽医》2020年第9期2917-2925,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划专项项目“畜禽重要病原耐药性检测与控制技术”(2016YFD0501302)。

摘　　要：为建立3种动物专用抗菌药(氟苯尼考、安普霉素和达氟沙星)在鸡肠道沙门菌的流行病学临界值(ECOFFs),本研究选取2017年分离自广东省各地兽医站和养殖场的166株鸡肠道沙门菌,采用琼脂稀释法测定3种动物专用抗菌药的最小抑菌浓度(MIC),利用非线性回归分析法及NRI(normalized resistance interpretation)和ECOFFinder程序分析处理MIC数据,并最终确定氟苯尼考、安普霉素和达氟沙星对鸡肠道沙门菌的ECOFFs。结果显示,氟苯尼考、安普霉素和达氟沙星对沙门菌的MIC范围分别为2~>512、4~>512和0.015~64μg/mL;MIC50和MIC90分别为256和>512、16和>512、0.5和16μg/mL。氟苯尼考和安普霉素的MIC值呈明显的单峰或双峰分布,且界限明显,而达氟沙星的MIC值出现了不连续多峰分布。综合3种分析方法,最终确定氟苯尼考、安普霉素和达氟沙星的ECOFFs分别为16、16和0.125μg/mL。本研究初步得到了3种药物在鸡肠道沙门菌的ECOFFs,为沙门菌耐药判定标准的制定提供了参考。In order to establish epidemiological cut-off values(ECOFFs)of Salmonella for three animal-specific antibiotics(florfenicol,apramycin and danofloxacin)in Guangdong province.A total of 166 strains of Salmonella isolates from chicken intestinal tract from veterinary clinics and farms in Guangdong province in 2017 were recruited in this study,the minimum inhibitory concentrations(MIC)of florfenicol,apramycin and danofloxacin were determined in triplicate for each bacterial strain using the agar dilution method on Mueller-Hinton agar plates according to the CLSI reference method(CLSI-M100-S26).ECOFFs were calculated for the MIC data sets by application of nonlinear regression analysis,NRI(normalized resistance interpretation)and ECOFFinder software.MIC distribution of florfenicol,apramycin and danofloxacin against Salmonella from 2 to>512,4 to>512 and 0.015 to 64μg/mL,respectively.MIC50 and MIC90 for apramycin,florfenicol and danofloxacin were 256 and>512,16 and>512,0.5 and 16μg/mL,respectively.MIC distribution of apramycin and florfenicol presented obvious unimodal shape,while that of danofloxacin was a discontinuous multi-peak.The ECOFFs of florfenicol,apramycin and danofloxacin were recommended to be 16,16 and 0.125μg/mL,respectively.In present study,the ECOFFs of Salmonella from chicken intestinal tract for apramycin,florfenicol and danofloxacin were determined,which provided scientific basis for resistance surveillance.

关 键 词：兽药 最小抑菌浓度(MIC) 沙门菌 流行病学临界值(ECOFFs) 

分 类 号：S852.612[农业科学—基础兽医学]